Heterologous production of bioactive xenoacremone analogs in Aspergillus nidulans.
10.1016/S1875-5364(23)60412-1
- Author:
Zhiguo LIU
1
,
2
;
Wei LI
1
,
3
;
Peng ZHANG
4
;
Yi SUN
5
;
Wen-Bing YIN
1
,
6
Author Information
1. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
3. Savaid Medical School, University of Chinese Academy of Sciences, Beijing 100049, China.
4. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
5. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. Electronic address: ysun@icmm.ac.cn.
6. Savaid Medical School, University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: yinwb@im.ac.cn.
- Publication Type:Journal Article
- Keywords:
Heterologous expression;
Natural products;
PKS-NRPS;
Tyrosine-decahydrofluorene;
Xenoacremones
- MeSH:
Aspergillus nidulans/metabolism*;
Biological Products/metabolism*;
Polyketides/metabolism*;
Peptides/metabolism*;
Biosynthetic Pathways;
Multigene Family
- From:
Chinese Journal of Natural Medicines (English Ed.)
2023;21(6):436-442
- CountryChina
- Language:English
-
Abstract:
Tyrosine-decahydrofluorene derivatives are a class of hybrid compounds that integrate the properties of polyketides and nonribosomal peptides. These compounds feature a [6.5.6] tricarbocyclic core and a para-cyclophane ether moiety in their structures and exhibit anti-tumor and anti-microbial activities. In this study, we constructed the biosynthetic pathway of xenoacremones from Xenoacremonium sinensis ML-31 in the Aspergillus nidulans host, resulting in the identification of four novel tyrosine-decahydrofluorene analogs, xenoacremones I-L (1-4), along with two known analogs, xenoacremones A and B. Remarkably, compounds 3 and 4 contained a 12-membered para-cyclophane ring system, which is unprecedented among tyrosine-decahydrofluorene analogs in X. sinensis. The successful reconstruction of the biosynthetic pathway and the discovery of novel analogs demonstrate the utility of heterologous expression strategy for the generation of structurally diverse natural products with potential biological activities.
