Potentilla anserina polysaccharide alleviates cadmium-induced oxidative stress and apoptosis of H9c2 cells by regulating the MG53-mediated RISK pathway.
10.1016/S1875-5364(23)60436-4
- Author:
Lixia ZHAO
1
,
2
,
3
;
Ju CHENG
4
;
Di LIU
5
;
Hongxia GONG
6
;
Decheng BAI
7
;
Wei SUN
8
Author Information
1. Institute of Integrated Traditional Chinese and Western Medicine, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China
2. School of Nursing, Gansu University of Chinese Medicine, Lanzhou 730000, China
3. Key Laboratory of Dunhuang Medicine, Ministry of Education, Gansu University of Chinese Medicine, Lanzhou 730000, China.
4. Institute of Genetics, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China.
5. Key laboratory of Evidence Science Techniques Research and Application of Gansu Province, Gansu University of Political Science and Law, Lanzhou 730000, China.
6. School of Basic Medical Sciences, Gansu University of Chinese Medicine, Lanzhou 730000, China.
7. Institute of Integrated Traditional Chinese and Western Medicine, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China.
8. Department of Cardiac Surgery, The First Hospital of Lanzhou University, Lanzhou 730000, China. Electronic address: swlz2022@163.com.
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Cadmium;
MG53;
Oxidative stress;
Polysaccharide;
Potentilla anserinaL.;
RISK pathway
- MeSH:
Cadmium/metabolism*;
Caspase 3/metabolism*;
Potentilla/metabolism*;
Glycogen Synthase Kinase 3 beta/pharmacology*;
Proto-Oncogene Proteins c-akt/metabolism*;
Cytochromes c/metabolism*;
Glutathione Disulfide/pharmacology*;
Oxidative Stress;
Myocytes, Cardiac;
Reactive Oxygen Species/metabolism*;
Reperfusion Injury/metabolism*;
Apoptosis;
Polysaccharides/pharmacology*;
Adenosine Triphosphate/metabolism*
- From:
Chinese Journal of Natural Medicines (English Ed.)
2023;21(4):279-291
- CountryChina
- Language:English
-
Abstract:
Oxidative stress plays a crucial role in cadmium (Cd)-induced myocardial injury. Mitsugumin 53 (MG53) and its mediated reperfusion injury salvage kinase (RISK) pathway have been demonstrated to be closely related to myocardial oxidative damage. Potentilla anserina L. polysaccharide (PAP) is a polysaccharide with antioxidant capacity, which exerts protective effect on Cd-induced damage. However, it remains unknown whether PAP can prevent and treat Cd-induced cardiomyocyte damages. The present study was desgined to explore the effect of PAP on Cd-induced damage in H9c2 cells based on MG53 and the mediated RISK pathway. For in vitro evaluation, cell viability and apoptosis rate were analyzed by CCK-8 assay and flow cytometry, respectively. Furthermore, oxidative stress was assessed by 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) staining and using superoxide dismutase (SOD), catalase (CAT), and glutathione/oxidized glutathione (GSH/GSSG) kits. The mitochondrial function was measured by JC-10 staining and ATP detection assay. Western blot was performed to detect the expression of proteins related to MG53, the RISK pathway, and apoptosis. The results indicated that Cd increased the levels of reactive oxygen species (ROS) in H9c2 cells. Cd decreased the activities of SOD and CAT and the ratio of GSH/GSSG, resulting in decreases in cell viability and increases in apoptosis. Interestingly, PAP reversed Cd-induced oxidative stress and cell apoptosis. Meanwhile, Cd reduced the expression of MG53 in H9c2 cells and inhibited the RISK pathway, which was mediated by decreasing the ratio of p-AktSer473/Akt, p-GSK3βSer9/GSK3β and p-ERK1/2/ERK1/2. In addition, Cd impaired mitochondrial function, which involved a reduction in ATP content and mitochondrial membrane potential (MMP), and an increase in the ratio of Bax/Bcl-2, cytoplasmic cytochrome c/mitochondrial cytochrome c, and Cleaved-Caspase 3/Pro-Caspase 3. Importantly, PAP alleviated Cd-induced MG53 reduction, activated the RISK pathway, and reduced mitochondrial damage. Interestingly, knockdown of MG53 or inhibition of the RISK pathway attenuated the protective effect of PAP in Cd-induced H9c2 cells. In sum, PAP reduces Cd-induced damage in H9c2 cells, which is mediated by increasing MG53 expression and activating the RISK pathway.
