Qianjin Wenwu decoction suppresses renal interstitial fibrosis by enhancing the degradation of extracellular matrix in mice with unilateral ureteral obstruction.
10.1016/S1875-5364(23)60434-0
- Author:
Chengshan JIN
1
,
2
;
Xiaotian WU
3
;
Yue YOU
3
;
Yuing WANG
4
;
Jing WU
4
;
Along ZUO
1
,
5
;
Yan ZHENG
6
;
Jianpeng GUO
1
,
7
Author Information
1. Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, College of Pharmacy, Yanbian University, Yanji 133002, China
2. Yanbian Chaoyao Biotechnology Co., Ltd., Yanji 133002, China.
3. Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, College of Pharmacy, Yanbian University, Yanji 133002, China.
4. Yanbian University Hospital, Yanji 133002, China.
5. Yanbian Medical and Health Industry Pilot Base, College of Pharmacy, Yanbian University, Yanji 133002, China.
6. Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, College of Pharmacy, Yanbian University, Yanji 133002, China. Electronic address: zhengyan_0308@163.com.
7. Yanbian Medical and Health Industry Pilot Base, College of Pharmacy, Yanbian University, Yanji 133002, China. Electronic address: gjp807@ybu.edu.cn.
- Publication Type:Journal Article
- Keywords:
Diabetic kidney disease (DKD);
Extracellular matrix (ECM);
Molecular docking;
Pharmacodyamic material basis;
QianJin Wenwu Decoction (QWD);
TGF-β1
- MeSH:
Rats;
Mice;
Animals;
Ureteral Obstruction/metabolism*;
Kidney/metabolism*;
Tissue Inhibitor of Metalloproteinase-1/metabolism*;
Molecular Docking Simulation;
Rats, Sprague-Dawley;
Kidney Diseases/drug therapy*;
Extracellular Matrix/metabolism*;
Flavonoids/metabolism*;
Fibrosis
- From:
Chinese Journal of Natural Medicines (English Ed.)
2023;21(4):253-262
- CountryChina
- Language:English
-
Abstract:
Diabetic kidney disease (DKD) is the most common complication of diabetes mellitus (DM). Qianjin Wenwu decoction (QWD), a well-known traditional Korean medicine, has been used for the treatment of DKD, with satisfactory therapeutic effects. This study was designed to investigate the active components and mechanisms of action of QWD in the treatment of DKD. The results demonstrated that a total of 13 active components in five types were found in QWD, including flavonoids, flavonoid glycosides, phenylpropionic acids, saponins, coumarins, and lignins. Two key proteins, TGF-β1 and TIMP-1, were identified as the target proteins through molecular docking. Furthermore, QWD significantly suppressed Scr and BUN levels which increased after unilateral ureteral obstruction (UUO). Hematoxylin & eosin (H&E) and Masson staining results demonstrated that QWD significantly alleviated renal interstitial fibrosis in UUO mice. We also found that QWD promoted ECM degradation by regulating MMP-9/TIMP-1 homeostasis to improve renal tubulointerstitial fibrosis and interfere with the expression and activity of TGF- β1 in DKD treatment. These findings explain the underlying mechanism of QWD for the treatment of DKD, and also provide methodological reference for investigating the mechanism of traditional medicine in the treatment of DKD.
