Physiological and transcriptional responses to heat stress in a typical phenotype of Pinellia ternata.
10.1016/S1875-5364(23)60433-9
- Author:
Jialu WANG
1
;
Jialei CHEN
2
;
Xiangyu ZHANG
3
;
Xue FENG
4
;
Xiwen LI
5
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
2. College of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, China.
3. Bijie Institute of Traditional Chinese Medicine, Bijie 551700, China.
4. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. Electronic address: xfeng0413@icmm.ac.cn.
5. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. Electronic address: xwli@icmm.ac.cn.
- Publication Type:Journal Article
- Keywords:
Chloroplast;
Heat stress;
Photosynthetic rate;
Pinellia ternata;
SOD and POD activities;
Transcriptional response
- MeSH:
Pinellia/genetics*;
Heat-Shock Response/genetics*;
Photosynthesis/genetics*;
Plants, Medicinal/genetics*;
Phenotype
- From:
Chinese Journal of Natural Medicines (English Ed.)
2023;21(4):243-252
- CountryChina
- Language:English
-
Abstract:
Pinellia ternata is an important medicinal plant, and its growth and development are easily threatened by high temperature. In this study, comprehensive research on physiological, cytological and transcriptional responses to different levels of heat stress were conducted on a typical phenotype of P. ternata. First, P. ternata exhibited tolerance to the increased temperature, which was supported by normal growing leaves, as well as decreased and sustained photosynthetic parameters. Severe stress aggravated the damages, and P. ternata displayed an obvious leaf senescence phenotype, with significantly increased SOD and POD activities (46% and 213%). In addition, mesophyll cells were seriously damaged, chloroplast thylakoid was fuzzy, grana lamellae and stroma lamellae were obviously broken, and grana thylakoids were stacked, resulting in a dramatically declined photosynthetic rate (74.6%). Moreover, a total of 16 808 genes were significantly differential expressed during this process, most of which were involved in photosynthesis, transmembrane transporter activity and plastid metabolism. The number of differentially expressed transcription factors in MYB and bHLH families was the largest, indicating that these genes might participate in heat stress response in P. ternata. These findings provide insight into the response to high temperature and facilitate the standardized cultivation of P. ternata.
