Single-cell transcriptomic atlas of mouse cochlear aging.

Guoqiang Sun; Yandong Zheng; Xiaolong FU; Weiqi Zhang; Jie Ren; Shuai MA; Shuhui Sun; Xiaojuan HE; Qiaoran Wang; Zhejun JI; Fang Cheng; Kaowen Yan; Ziyi Liu; Juan Carlos Izpisua Belmonte; Jing QU; Si Wang; Renjie Chai; Guang-hui Liu

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Single-cell transcriptomic atlas of mouse cochlear aging.

Author: Guoqiang SUN ¹ ; Yandong ZHENG ¹ ; Xiaolong FU ² ; Weiqi ZHANG ³ ; Jie REN ³ ; Shuai MA ⁴ ; Shuhui SUN ⁴ ; Xiaojuan HE ⁵ ; Qiaoran WANG ³ ; Zhejun JI ¹ ; Fang CHENG ⁶ ; Kaowen YAN ⁴ ; Ziyi LIU ⁷ ; Juan Carlos Izpisua BELMONTE ⁸ ; Jing QU ¹ ; Si WANG ⁵ ; Renjie CHAI ² ; Guang-Hui LIU ⁴
Author Information

1. State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
2. State Key Laboratory of Bioelectronics, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, School of Life Sciences and Technology, Southeast University, Nanjing 211189, China.
3. CAS Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China.
4. State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
5. Advanced Innovation Center for Human Brain Protection, National Clinical Research Center for Geriatric Disorders, Xuanwu Hospital Capital Medical University, Beijing 100053, China.
6. National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
7. Shandong Provincial Hospital and School of Laboratory Animal Science, Shandong First Medical University, Jinan 250000, China.
8. Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
Publication Type:Research Support, Non-U.S. Gov't
Keywords: aging; cochlea; mouse; single-cell transcriptomic atlas
MeSH: Mice; Animals; Transcriptome; Aging/metabolism*; Cochlea; Stria Vascularis; Presbycusis
From: Protein & Cell 2023;14(3):180-201
CountryChina
Language:English
Abstract: Progressive functional deterioration in the cochlea is associated with age-related hearing loss (ARHL). However, the cellular and molecular basis underlying cochlear aging remains largely unknown. Here, we established a dynamic single-cell transcriptomic landscape of mouse cochlear aging, in which we characterized aging-associated transcriptomic changes in 27 different cochlear cell types across five different time points. Overall, our analysis pinpoints loss of proteostasis and elevated apoptosis as the hallmark features of cochlear aging, highlights unexpected age-related transcriptional fluctuations in intermediate cells localized in the stria vascularis (SV) and demonstrates that upregulation of endoplasmic reticulum (ER) chaperon protein HSP90AA1 mitigates ER stress-induced damages associated with aging. Our work suggests that targeting unfolded protein response pathways may help alleviate aging-related SV atrophy and hence delay the progression of ARHL.