Chemical constituents from leaves of Jatropha curcas.
10.1016/j.chmed.2022.08.010
- Author:
Yingjie WANG
1
;
Di ZHOU
1
;
Xiaolin BAI
2
;
Qingqi MENG
3
;
Haihui XIE
4
;
Guojiang WU
4
;
Gang CHEN
1
;
Yue HOU
3
;
Ning LI
1
Author Information
1. School of Traditional Chinese Materia Medica, Key Laboratory of Innovative Traditional Chinese Medicine for Major Chronic Diseases of Liaoning Province, Key Laboratory for TCM Material Basis Study and Innovative Drug Development of Shenyang City, Shenyang Pharmaceutical University, Shenyang 110016, China.
2. Tasly Northeast Modern TCM Resources Co., Ltd., Benxi 117204, China.
3. College of Life and Health Sciences, Northeastern University, Shenyang 110004, China.
4. South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
- Publication Type:Journal Article
- Keywords:
Jatropha curcas L.;
anti-neuroinflammation;
flavonoid;
lignans
- From:
Chinese Herbal Medicines
2023;15(3):463-469
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To investigate the chemical constituents from the leaves of Jatropha curcas and evaluate their inhibition on lipopolysaccharide (LPS)-activated BV-2 microglia cells.
METHODS:The n-BuOH extract of the leaves of J. curcas was isolated by macroporous adsorption resin, silica gel, ODS, column chromatography and semi-preparative HPLC. The structures of the compounds were identified by MS, NMR, ECD, and other spectroscopic methods. In addition, anti-neuroinflammatory effects of isolated compounds were evaluated by measuring the production of nitric oxide (NO) in over-activated BV-2 cells.
RESULTS:Seventeen compounds, including (7R,8S)-crataegifin A-4-O-β-D-glucopyranoside ( 1), (8R,8'R)-arctigenin ( 2), arctigenin-4'-O-β-D-glucopyranoside ( 3), (-)-syringaresinol ( 4), syringaresinol-4'-O-β-D-glucopyranoside ( 5), (-)-pinoresinol ( 6), pinoresinol-4'-O-β-D-glucopyranoside ( 7), buddlenol D ( 8), (2R,3R)-dihydroquercetin ( 9), (2S,3S)-epicatechin ( 10), (2R,3S)-catechin ( 11), isovitexin ( 12), naringenin-7-O-β-D-glucopyranoside ( 13), chamaejasmin ( 14), neochamaejasmin B ( 15), isoneochamaejasmin A ( 16), and tomentin-5-O-β-D-glucopyranoside ( 17) were isolated and identified. Compounds 2, 4 and 8 significantly inhibited the release of NO in BV-2 microglia activated by LPS, with IC50 values of 18.34, 29.33 and 26.30 μmol/L, respectively.
CONCLUSION:Compound 1 is a novel compound, and compounds 2, 3, 8, 14- 17 are isolated from Jatropha genus for the first time. In addition, the lignans significantly inhibited NO release and the inhibitory activity was decreased after glycosylation.
