Chemical profiling and rapid discrimination of Blumea riparia and Blumea megacephala by UPLC-Q-Exactive-MS/MS and HPLC.
10.1016/j.chmed.2022.06.009
- Author:
Hongna SU
1
;
Xuexue LI
1
;
Ying LI
1
;
Yuanlin KONG
1
;
Jianlong LAN
1
;
Yanfei HUANG
2
;
Yuan LIU
2
Author Information
1. College of Pharmacy, Southwest Minzu University, Chengdu 610225, China.
2. Key Laboratory of Qinghai-Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization of National Ethnic Affairs Commission, Chengdu 610225, China.
- Publication Type:Journal Article
- Keywords:
Blumea megacephala (Randeria) Chang et Tseng;
Blumea riparia (Bl.) DC.;
UPLC-Q-Exactive-MS/MS;
chemical pattern recognition
- From:
Chinese Herbal Medicines
2023;15(2):317-328
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To rapidly identify the two morphologies and chemical properties of similar herbal medicines, Blumea riparia and B. megacephala as the basis for chemical constituent analysis.
METHODS:UPLC-Q-Exactive-MS/MS was utilized for profiling and identification of the constituents in B. riparia and B. megacephala. Chemical pattern recognition (CPR) was further used to compare and distinguish the two herbs and to identify their potential characteristic markers. Then, an HPLC method was established for quality evaluation.
RESULTS:A total of 93 constituents are identified, including 54 phenolic acids, 35 flavonoids, two saccharides, one phenolic acid glycoside, and one other constituent, of which 67 were identified in B. riparia and B. megacephala for the first time. CPR indicates that B. riparia and B. megacephala samples can be distinguished from each other based on the LC-MS data. The isochlorogenic acid A to cryptochlorogenic acid peak area ratio calculated from the HPLC chromatograms was proposed as a differentiation index for distinguishing and quality control of B. riparia and B. megacephala.
CONCLUSION:This study demonstrates significant differences between B. riparia and B. megacephala in terms of chemical composition. The results provide a rapid and simple strategy for the comparison and evaluation of the quality of B. riparia and B. megacephala.
