Chloroplast DNA reveals genetic population structure in Sinomenium acutum in subtropical China.
10.1016/j.chmed.2022.11.003
- Author:
Chun GUO
1
;
Ying HE
1
;
Xiyao ZENG
2
;
Xingyao XIONG
3
;
Ping QIU
4
;
Xueshuang HUANG
5
;
Hua YANG
2
Author Information
1. Center for Medical Research and Innovation, The First Hospital of Hunan University of Chinese Medicine, Changsha 410007, China.
2. College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410125, China.
3. College of Horticulture and Landscape, Hunan Agricultural University, Changsha 410125, China.
4. Hunan Zhengqing Pharmaceutical Group Co., Ltd., Huaihua 418000, China.
5. Hunan Provincial Key Laboratory for Synthetic Biology of Traditional Chinese Medicine, Hunan University of Medicine, Huaihua 418000, China.
- Publication Type:Journal Article
- Keywords:
Sinomenium acutum (Thunb.) Rehd. et Wils;
chloroplast DNA;
genetic diversity;
phylogeny;
population variation
- From:
Chinese Herbal Medicines
2023;15(2):278-283
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:The population density and diversity of Sinomenium acutum (Menispermaceae) have been greatly reduced recently by overharvesting for medicinal purposes in China. Therefore, it is urgent that the remaining populations are investigated, and that strategies for the utilization and conservation of this species are developed. This study aimed to find the possible glacial refugia and define the genetic diversity of S. acutum for its proper utilization and conservation.
METHODS:A total of 77 S. acutum samples were collected from four locations, Qinling Mountains, Daba Mountains, Dalou Mountains, and Xuefeng Mountains, in subtropical China. Genetic diversity among and between these populations were phylogenetically analyzed using four chloroplast DNA molecular markers (atpI-atpH, trnQ-5'rps16, trnH-psbA and trnL-trnF).
RESULTS:A total of 14 haplotypes (C1 to C14) were found in collected samples. Haplotypes C1 and C3 were shared among all populations, with C3 as the ancestral haplotype. Haplotypes C11 and C12 diverged the most from C3 and other haplotypes. No obvious phylogeographic structure was found in four locations using the GST/NST test. There is no evidence of rapid demographic expansion in S. acutum based on the mismatch distribution, and the results of Tajima's D test, and Fu's FS test. Our analyses of molecular variance revealed a high level of genetic variation within populations. In contrast, the genetic differentiation among S. acutum populations was low, indicating frequent gene flow.
CONCLUSION:Xuefeng, Dalou, and Daba Mountains were possible glacial refugia for the populations of S. acutum. C1, C3, C11 and C12 haplotypes of S. acutum should be carefully preserved and managed for their genetic value.
