RANKL+ senescent cells under mechanical stress: a therapeutic target for orthodontic root resorption using senolytics.
10.1038/s41368-023-00228-1
- Author:
Yue ZHOU
1
;
Aki NISHIURA
2
;
Hidetoshi MORIKUNI
1
;
Wenqi DENG
1
;
Toru TSUJIBAYASHI
3
;
Yoshihiro MOMOTA
4
;
Yuki AZETSU
5
;
Masamichi TAKAMI
5
;
Yoshitomo HONDA
6
;
Naoyuki MATSUMOTO
1
Author Information
1. Department of Orthodontics, Osaka Dental University, 8-1 Kuzuhahanazonocho, Hirakata, Osaka, Japan.
2. Department of Orthodontics, Osaka Dental University, 8-1 Kuzuhahanazonocho, Hirakata, Osaka, Japan. nishiura@cc.osaka-dent.ac.jp.
3. Department of Physics, Osaka Dental University, 8-1 Kuzuhahanazonocho, Hirakata, Osaka, Japan.
4. Department of Anesthesiology, Osaka Dental University, 8-1 Kuzuhahanazonocho, Hirakata, Osaka, Japan.
5. Department of Pharmacology, Showa University School of Dentistry, 1-5-8 Hatanodai, Shinagawaku, Tokyo, Japan.
6. Department of Oral Anatomy, Osaka Dental University, 8-1 Kuzuhahanazonocho, Hirakata, Osaka, Japan. honda-y@cc.osaka-dent.ac.jp.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Rats;
Animals;
Root Resorption/prevention & control*;
Senotherapeutics;
Stress, Mechanical;
Dasatinib/pharmacology*;
Quercetin/pharmacology*;
Osteoclasts;
Tooth Movement Techniques;
Periodontal Ligament;
RANK Ligand
- From:
International Journal of Oral Science
2023;15(1):20-20
- CountryChina
- Language:English
-
Abstract:
In dentistry, orthodontic root resorption is a long-lasting issue with no effective treatment strategy, and its mechanisms, especially those related to senescent cells, remain largely unknown. Here, we used an orthodontic intrusion tooth movement model with an L-loop in rats to demonstrate that mechanical stress-induced senescent cells aggravate apical root resorption, which was prevented by administering senolytics (a dasatinib and quercetin cocktail). Our results indicated that cementoblasts and periodontal ligament cells underwent cellular senescence (p21+ or p16+) and strongly expressed receptor activator of nuclear factor-kappa B (RANKL) from day three, subsequently inducing tartrate-resistant acid phosphatase (TRAP)-positive odontoclasts and provoking apical root resorption. More p21+ senescent cells expressed RANKL than p16+ senescent cells. We observed only minor changes in the number of RANKL+ non-senescent cells, whereas RANKL+ senescent cells markedly increased from day seven. Intriguingly, we also found cathepsin K+p21+p16+ cells in the root resorption fossa, suggesting senescent odontoclasts. Oral administration of dasatinib and quercetin markedly reduced these senescent cells and TRAP+ cells, eventually alleviating root resorption. Altogether, these results unveil those aberrant stimuli in orthodontic intrusive tooth movement induced RANKL+ early senescent cells, which have a pivotal role in odontoclastogenesis and subsequent root resorption. These findings offer a new therapeutic target to prevent root resorption during orthodontic tooth movement.
