In vivo antioxidant activity of rabbiteye blueberry (<i>Vaccinium ashei</i> cv. 'Brightwell') anthocyanin extracts.

Jing Wang; Xingyu Zhao; Jiawei Zheng; Daniela D Herrera-balandrano; Xiaoxiao Zhang; Wuyang Huang; Zhongquan Sui

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In vivo antioxidant activity of rabbiteye blueberry (Vaccinium ashei cv. 'Brightwell') anthocyanin extracts.

Author: Jing WANG ¹ ; Xingyu ZHAO ² ; Jiawei ZHENG ² ; Daniela D HERRERA-BALANDRANO ³ ; Xiaoxiao ZHANG ² ; Wuyang HUANG ⁴ ; Zhongquan SUI ⁵
Author Information

1. College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
2. Institute of Agro-Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
3. School of Life Sciences, Nantong University, Nantong 226007, China.
4. College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China. wuyanghuang@hotmail.com.
5. Department of Food Science & Engineering, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China. wuyanghuang@hotmail.com, zsui@sjtu.edu.cn.
Publication Type:Journal Article
Keywords: Blueberry anthocyanin; Glutathione-peroxidase (GSH-PX/GPX); In vivo antioxidant activity; Malondialdehyde (MDA); Superoxide dismutase (SOD)
MeSH: Male; Mice; Animals; Antioxidants/pharmacology*; Blueberry Plants; Anthocyanins/pharmacology*; Mice, Inbred C57BL; Superoxide Dismutase; Plant Extracts/pharmacology*; Superoxide Dismutase-1
From: Journal of Zhejiang University. Science. B 2023;24(7):602-616
CountryChina
Language:English
Abstract: Blueberries are rich in phenolic compounds including anthocyanins which are closely related to biological health functions. The purpose of this study was to investigate the antioxidant activity of blueberry anthocyanins extracted from 'Brightwell' rabbiteye blueberries in mice. After one week of adaptation, C57BL/6J healthy male mice were divided into different groups that were administered with 100, 400, or 800 mg/kg blueberry anthocyanin extract (BAE), and sacrificed at different time points (0.1, 0.5, 1, 2, 4, 8, or 12 h). The plasma, eyeball, intestine, liver, and adipose tissues were collected to compare their antioxidant activity, including total antioxidant capacity (T-AOC), superoxide dismutase (SOD) activity and glutathione-peroxidase (GSH-PX/GPX) content, and the oxidative stress marker malondialdehyde (MDA) level. The results showed that blueberry anthocyanins had positive concentration-dependent antioxidant activity in vivo. The greater the concentration of BAE, the higher the T-AOC value, but the lower the MDA level. The enzyme activity of SOD, the content of GSH-PX, and messenger RNA (mRNA) levels of Cu,Zn-SOD, Mn-SOD, and GPX all confirmed that BAE played an antioxidant role after digestion in mice by improving their antioxidant defense. The in vivo antioxidant activity of BAE indicated that blueberry anthocyanins could be developed into functional foods or nutraceuticals with the aim of preventing or treating oxidative stress-related diseases.