Ultrashort wave alleviates oxygen -glucose deprivation/reoxygenation injury via up -regulation of SPCA1 expression in N2a cells.
10.11817/j.issn.1672-7347.2023.220510
- Author:
Jinling TANG
1
;
Rumi WANG
2
;
Yongmei FAN
3
;
Changjie ZHANG
2
;
Ying KONG
2
Author Information
1. Department of Rehabilitation Medicine, Second Xiangya Hospital, Central South University, Changsha 410011, China. 2602856130@qq.com.
2. Department of Rehabilitation Medicine, Second Xiangya Hospital, Central South University, Changsha 410011, China.
3. Department of Rehabilitation Medicine, Second Xiangya Hospital, Central South University, Changsha 410011, China. fanyongmei@csu.edu.cn.
- Publication Type:Journal Article
- Keywords:
Golgi apparatus stress;
apoptosis;
oxygen-glucose deprivation/reperfusion;
secretory pathway Ca 2+-ATPase 1;
ultrashort wave
- MeSH:
Animals;
Rats;
Apoptosis;
Brain Ischemia;
Glucose/metabolism*;
Oxygen/metabolism*;
Reperfusion Injury/metabolism*;
Transcriptional Activation;
Up-Regulation;
Calcium-Transporting ATPases/metabolism*
- From:
Journal of Central South University(Medical Sciences)
2023;48(5):641-647
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:Application of ultrashort wave (USW) to rats with cerebral ischemia and reperfusion injury could inhibit the decrease of expression of secretory pathway Ca2+-ATPase 1 (SPCA1), an important participant in Golgi stress, reduce the damage of Golgi apparatus and the apoptosis of neuronal cells, thereby alleviating cerebral ischemia-reperfusion injury. This study aims to investigate the effect of USW on oxygen-glucose deprivation/reperfusion (OGD/R) injury and the expression of SPCA1 at the cellular level.
METHODS:N2a cells were randomly divided into a control (Con) group, an OGD/R group, and an USW group. The cells in the Con group were cultured without exposure to OGD. The cells in the OGD/R group were treated with OGD/R. The cells in the USW group were treated with USW after OGD/R. Cell morphology was observed under the inverted phase-contrast optical microscope, cell activity was detected by cell counting kit-8 (CCK-8), apoptosis was detected by flow cytometry, and SPCA1 expression was detected by Western blotting.
RESULTS:Most of the cells in the Con group showed spindle shape with a clear outline and good adhesion. In the OGD/R group, cells were wrinkled, with blurred outline, poor adhesion, and lots of suspended dead cells appeared; compared with the OGD/R group, the cell morphology and adherence were improved, with clearer outlines and fewer dead cells in the USW group. Compared with the Con group, the OGD/R group showed decreased cell activity, increased apoptotic rate, and down-regulating SPCA1 expression with significant differences (all P<0.001); compared with the OGD/R group, the USW group showed increased cell activity, decreased apoptotic rate, and up-regulating SPCA1 expression with significant differences (P<0.01 or P<0.001).
CONCLUSIONS:USW alleviates the injury of cellular OGD/R, and its protective effect may be related to its up-regulation of SPCA1 expression.
