Effects of C10orf10 on growth and prognosis of glioma under hypoxia.
10.11817/j.issn.1672-7347.2023.220396
- Author:
Yuanbing CHEN
1
;
Miao TANG
2
;
Hui LI
3
;
Jun HUANG
4
Author Information
1. Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha 410008. chenyuanbing@csu.edu.cn.
2. Department of Neurosurgery, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510000, China.
3. Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha 410008.
4. Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha 410008. xyyyhj@csu.edu.cn.
- Publication Type:Journal Article
- Keywords:
C10orf10 gene;
glioma;
hypoxia;
prognosis
- MeSH:
Humans;
Central Nervous System;
Glioma/genetics*;
Hypoxia;
Neoplasm Recurrence, Local;
Prognosis;
Tumor Microenvironment
- From:
Journal of Central South University(Medical Sciences)
2023;48(4):499-507
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:Glioma is the most common malignant tumor in the central nervous system, and the hypoxic microenvironment is prevalent in solid tumors. This study aims to investigate the up-regulation of genes under the condition of hypoxia and their roles in glioma growth, as well as their impact on glioma prognosis.
METHODS:The hypoxia-related dataset with glioma was screened in the Gene Expression Omnibus database (GEO), and the differentially expressed genes were analyzed between hypoxia and normoxia through bioinformatics, and chromosome 10 open reading frame 10 (C10orf10) was verified and screened in hypoxia-treated cells through real-time PCR and Western blotting. The Cancer Genome Atlas (TCGA) and the Chinese Glioma Genome Atlas (CGGA) datasets were downloaded to analyze the mRNA expression of C10orf10 in different grades of glioma and its impact on prognosis. The glioma specimens and follow-up data of 68 gliomas who underwent surgical treatment in Xiangya Hospital of Central South University from March 2017 to January 2021 were collected, and real-time PCR was used to detect the mRNA expression of C10orf10 in different grades of glioma, and the Kaplan-Meier method was used to analyze the relationship between the expression C10orf10 and prognosis. The glioma cells, which could interfere the expression of C10orf10, were constructed, and the effect of C10orf10 on the proliferation of glioma cells was evaluated by cell counting kit-8 (CCK-8) and colony formation assays.
RESULTS:Compared with the condition of normoxia, the expression levels of C10orf10 mRNA and protein were significantly up-regulated in glioma cells under hypoxia (P<0.001), and the mRNA expression level of C10orf10 in glioma tissues was up-regulated with the increase of WHO grade in glioma (P<0.001). Based on Kaplan-Meier survival analysis, the higher the mRNA expression level of C10orf10 was, the shorter the survival time of the patient was (P<0.05). And the expression of C10orf10 mRNA was higher in recurrent gliomas than that in primary gliomas in the CGGA database (P<0.001). Knockdown of C10orf10 could significantly inhibit the growth of glioma cells both under hypoxia and normoxia (both P<0.001).
CONCLUSIONS:The expression level of C10orf10 can promote the proliferation and prognosis of glioma, which is expected to become a prognostic marker and therapeutic target for glioma.
