Ethanol Extract of Glycyrrhiza uralensis Fisch: Antidiarrheal Activity in Mice and Contraction Effect in Isolated Rabbit Jejunum.
10.1007/s11655-022-3536-5
- Author:
Jing WEN
1
;
Jian-Wu ZHANG
1
;
Yuan-Xia LYU
1
;
Hui ZHANG
1
;
Kai-Xi DENG
1
;
Hong-Xue CHEN
1
;
Ying WEI
2
Author Information
1. Department of Pharmacy, North Sichuan Medical College, Nanchong, Sichuan Province, 637100, China.
2. Department of Pharmacy, North Sichuan Medical College, Nanchong, Sichuan Province, 637100, China. 414697669@qq.com.
- Publication Type:Journal Article
- Keywords:
Chinese medicine;
Glycyrrhiza uralensis Fisch;
antidiarrheal;
spasmolysis
- MeSH:
Mice;
Rabbits;
Animals;
Antidiarrheals/adverse effects*;
Jejunum;
Glycyrrhiza uralensis;
Castor Oil/adverse effects*;
Calcium Chloride/adverse effects*;
Diarrhea/drug therapy*;
Plant Extracts/adverse effects*;
Verapamil/adverse effects*;
Muscle Contraction
- From:
Chinese journal of integrative medicine
2023;29(4):325-332
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To evaluate the antidiarrheal effect of ethanol extract of Glycyrrhiza uralensis Fisch root (GFR) in vivo and jejunal contraction in vitro.
METHODS:In vivo, 50 mice were divided into negative control, positive control (verapamil), low-, medium- and high-dose GFR (250, 500, 1,000 mg/kg) groups by a random number table, 10 mice in each group. The antidiarrheal activity was evaluated in castor oil-induced diarrhea mice model by evacuation index (EI). In vitro, the effects of GFR (0.01, 0.03, 0.1, 0.3, 1, 3, and 10 g/L) on the spontaneous contraction of isolated smooth muscle of rabbit jejunum and contraction of pretreated by Acetylcholine (ACh, 10 µmol/L) and KCl (60 mmol/L) were observed for 200 s. In addition, CaCl2 was accumulated to further study its mechanism after pretreating jejunal smooth muscle with GFR (1 and 3 g/L) or verapamil (0.03 and 0.1 µmol/L) in a Ca2+-free-high-K+ solution containing ethylene diamine tetraacetic acid (EDTA).
RESULTS:GFR (500 and 1,000 mg/kg) significantly reduced EI in castor oil-induced diarrhea model mice (P<0.01). Meanwhile, GFR (0.01, 0.03, 0.1, 0.3, 1, 3, and 10 g/L) inhibited the spontaneous contraction of rabbit jejunum (P<0.05 or P<0.01). Contraction of jejunums samples pretreated by ACh and KCl with 50% effective concentration (EC50) values was 1.05 (0.71-1.24), 0.34 (0.29-0.41) and 0.15 (0.11-0.20) g/L, respectively. In addition, GFR moved the concentration-effect curve of CaCl2 down to the right, showing a similar effect to verapamil.
CONCLUSIONS:GFR can effectively against diarrhea and inhibit intestinal contraction, and these antidiarrheal effects may be based on blocking L-type Ca2+ channels and muscarinic receptors.
