Amygdalin Ameliorates Liver Fibrosis through Inhibiting Activation of TGF-β/Smad Signaling.
10.1007/s11655-021-3304-y
- Author:
Zhun XIAO
1
;
Qiang JI
1
;
Ya-Dong FU
1
;
Si-Qi GAO
1
;
Yong-Hong HU
1
;
Wei LIU
1
;
Gao-Feng CHEN
1
;
Yong-Ping MU
1
;
Jia-Mei CHEN
2
;
Ping LIU
1
Author Information
1. Institute of Liver Diseases, Key Laboratory of Liver and Kidney Diseases (Ministry of Education), Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.
2. Institute of Liver Diseases, Key Laboratory of Liver and Kidney Diseases (Ministry of Education), Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China. cjm0102@126.com.
- Publication Type:Journal Article
- Keywords:
TGF-β/Smad signaling pathway;
amygdalin;
hepatic stellate cells;
liver fibrosis;
liver sinusoidal endothelial cells
- MeSH:
Rats;
Male;
Mice;
Animals;
Transforming Growth Factor beta/metabolism*;
Amygdalin/therapeutic use*;
Endothelial Cells/metabolism*;
Olive Oil/therapeutic use*;
Rats, Wistar;
Smad Proteins/metabolism*;
Liver Cirrhosis/metabolism*;
Liver;
Transforming Growth Factor beta1/metabolism*;
Signal Transduction;
Collagen Type I/metabolism*;
Carbon Tetrachloride;
Hepatic Stellate Cells
- From:
Chinese journal of integrative medicine
2023;29(4):316-324
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To observe the effect of amygdalin on liver fibrosis in a liver fibrosis mouse model, and the underlying mechanisms were partly dissected in vivo and in vitro.
METHODS:Thirty-two male mice were randomly divided into 4 groups, including control, model, low- and high-dose amygdalin-treated groups, 8 mice in each group. Except the control group, mice in the other groups were injected intraperitoneally with 10% carbon tetrachloride (CCl4)-olive oil solution 3 times a week for 6 weeks to induce liver fibrosis. At the first 3 weeks, amygdalin (1.35 and 2.7 mg/kg body weight) were administered by gavage once a day. Mice in the control group received equal quantities of subcutaneous olive oil and intragastric water from the fourth week. At the end of 6 weeks, liver tissue samples were harvested to detect the content of hydroxyproline (Hyp). Hematoxylin and eosin and Sirius red staining were used to observe the inflammation and fibrosis of liver tissue. The expressions of collagen I (Col-I), alpha-smooth muscle actin (α-SMA), CD31 and transforming growth factor β (TGF-β)/Smad signaling pathway were observed by immunohistochemistry, quantitative real-time polymerase chain reaction and Western blot, respectively. The activation models of hepatic stellate cells, JS-1 and LX-2 cells induced by TGF-β1 were used in vitro with or without different concentrations of amygdalin (0.1, 1, 10 µmol/L). LSECs. The effect of different concentrations of amygdalin on the expressions of liver sinusoidal endothelial cells (LSECs) dedifferentiation markers CD31 and CD44 were observed.
RESULTS:High-dose of amygdalin significantly reduced the Hyp content and percentage of collagen positive area, and decreased the mRNA and protein expressions of Col-I, α-SMA, CD31 and p-Smad2/3 in liver tissues of mice compared to the model group (P<0.01). Amygdalin down-regulated the expressions of Col-I and α-SMA in JS-1 and LX-2 cells, and TGFβ R1, TGFβ R2 and p-Smad2/3 in LX-2 cells compared to the model group (P<0.05 or P<0.01). Moreover, 1 and 10 µmol/L amygdalin inhibited the mRNA and protein expressions of CD31 in LSECs and increased CD44 expression compared to the model group (P<0.05 or P<0.01).
CONCLUSIONS:Amygdalin can dramatically alleviate liver fibrosis induced by CCl4 in mice and inhibit TGF-β/Smad signaling pathway, consequently suppressing HSCs activation and LSECs dedifferentiation to improve angiogenesis.
