Chronic Injury of Mice Bone Marrow Multipotent Hematopoietic Progenitor Cells Induced by Ionizing Radiation.
10.19746/j.cnki.issn.1009-2137.2023.03.038
- Author:
Ying-Ying LUAN
1
,
2
;
Juan YANG
2
,
3
;
Shuo YANG
2
,
3
;
Han-Cheng FAN
1
,
2
;
Zi-Hao YANG
2
,
4
,
5
;
Jin-Fu ZHANG
2
,
4
,
5
;
Rong DENG
2
,
4
,
5
;
Hua WANG
2
,
6
;
Hui-Hong ZENG
1
,
7
;
Li-Jian SHAO
4
,
8
,
9
Author Information
1. Department of Histology and Embryology, School of Basic Medicine
2. Nanchang University, Nanchang 330006, Jiangxi Province, China.
3. Department of Occupational Health and Toxicology, School of Public Health,Nanchang University, Nanchang 330006, Jiangxi Province, China,Jiangxi Provincial Key Laboratory of Preventive Medicine, School of Public Health
4. Department of Occupational Health and Toxicology, School of Public Health
5. Nanchang University, Nanchang 330006, Jiangxi Province, China,Jiangxi Provincial Key Laboratory of Preventive Medicine, School of Public Health
6. Department of Oncology, Second Affiliated Hospital of Nanchang University
7. Nanchang University, Nanchang 330006, Jiangxi Province, China,Jiangxi Provincial Key Laboratory of Interdisciplinary Science，Nanchang University, Nanchang 330006, Jiangxi Province, China.
8. Nanchang University, Nanchang 330006, Jiangxi Province, China,Jiangxi Provincial Key Laboratory of Preventive Medicine, School of Public Health
9. Nanchang University, Nanchang 330006, Jiangxi Province, China,Jiangxi Provincial Key Laboratory of Interdisciplinary Science，Nanchang University, Nanchang 330006, Jiangxi Province, China,E-mail: lshao@ncu.edu.cn.
- Publication Type:Journal Article
- Keywords:
cell apoptosis;
cellular senescence;
ionizing radiation;
multipotent hematopoietic progenitor cells;
oxidative stress
- MeSH:
Mice;
Animals;
Bone Marrow;
Reactive Oxygen Species;
Mice, Inbred C57BL;
Hematopoietic Stem Cells;
Whole-Body Irradiation;
Radiation, Ionizing;
Bone Marrow Cells
- From:
Journal of Experimental Hematology
2023;31(3):871-879
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the chronic injury and its possible mechanism of ionizing radiation on multipotent hematopoietic progenitor cells (MPPs) by determining the related indicators of MPPs in bone marrow of mice post-radiation.
METHODS:Sixteen C57BL/6 adult mice were randomly divided into normal control and irradiation groups, 8 mice in each group. The mice in irradiation group were exposed to 6 Gy X-ray. The proportion of bone marrow MPPs, their apoptosis and proliferation 2 months after irradiation were detected by flow cytometry. Mitochondrial activity and levels of reactive oxygen species (ROS) in each MPPs population were detected by Mitotracker Red and DCFDA probes, and the senescent state of MPPs in the bone marrow was analyzed.
RESULTS:Ionizing radiation could reduce the proportion of MPPs in mouse bone marrow. The proportions and numbers of MPP1, MPP3 and MPP4 in the bone marrow were significantly decreased after whole-body irradiation with 6 Gy X-ray (P<0.05). In addition, radiation significantly reduced the colony-forming capacity of MPPs in bone marrow (P<0.05), the proportions of apoptotic cells in the MPP1 and MPP4 cell populations increased significantly in the bone marrow (P<0.05). The activity of mitochondria was significantly reduced in the bone marrow MPP2, MPP3 and MPP4 cell populations compared with that of the control group (P<0.05). It was also found that the radiation could significantly increase the ROS levels of MPPs in bone marrow, and the content of ROS in the MPP2, MPP3 and MPP4 cell population of the bone marrow was significantly increased(P<0.05). The senescent cells ratios of MPP1, MPP3 and MPP4 cells in the bone marrow after irradiation were significantly higher than those in the control group (P<0.05).
CONCLUSION:Ionizing radiation can cause chronic MPPs damage in mice, which is closely associated with persistent oxidative stress, cells apoptosis, and cellular senescence.
