Analysis of Gene Recombination between HLA-B and -DRB1, HLA-DQB1 and -DPB1 Loci.
10.19746/j.cnki.issn.1009-2137.2023.03.035
- Author:
Chen CHEN
1
,
2
,
3
;
Wei WANG
3
,
4
;
Nan-Ying CHEN
3
,
4
;
Li-Na DONG
3
,
4
;
Wei ZHANG
3
,
4
;
Fa-Ming ZHU
4
,
5
Author Information
1. School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang Province, China
2. Blood Center of Zhejiang Province, Hangzhou 310052, Zhejiang Province, China
3. Key Laboratory of Blood Safety Research of Zhejiang Province, Hangzhou 310052, Zhejiang Province, China.
4. Blood Center of Zhejiang Province, Hangzhou 310052, Zhejiang Province, China
5. Key Laboratory of Blood Safety Research of Zhejiang Province, Hangzhou 310052, Zhejiang Province, China.E-mail: zfm00@hotmail.com.
- Publication Type:Journal Article
- Keywords:
gene recombination;
human leukocyte antigen (HLA);
next-generation sequencing
- MeSH:
Humans;
Gene Frequency;
HLA-DQ beta-Chains/genetics*;
HLA-B Antigens/genetics*;
Histocompatibility Antigens Class I/genetics*;
Haplotypes;
HLA-A Antigens/genetics*;
HLA-DRB1 Chains/genetics*;
Recombination, Genetic;
Alleles
- From:
Journal of Experimental Hematology
2023;31(3):855-859
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the recombinations within the human leukocyte antigen (HLA) region in two families.
METHODS:Genomic DNA was extracted from the peripheral blood specimens of the different family members. HLA-A, -B, -C, -DRB1, -DQB1 and -DPB1 loci were genotyped using polymerase chain reaction-sequence specific oligonucleotide probing technique (PCR-SSO) and next-generation sequencing technique. HLA haplotype was determined by genetic analysis of the pedigree.
RESULTS:The haplotypes of HLA-A*11:01~C*03:04~B*13:01~DRB1*12:02~DQB1*03:01~DPB1*05:01:01G and HLA-A*03:01~C*04:01~B*35:03~DRB1*12:01~DQB1*03:01~DPB1*04:01:01G in the family 1 were recombined between HLA-B and HLA-DRB1 loci, which formed the haplotype of HLA-A*11:01~C*03:04~B*13:01~DRB1* 12:01~DQB1*03:01~DPB1*04:01:01G. The haplotypes of HLA-A *02:06~C*03:03~B*35:01~DRB1*08:02~DQB1*04:02~ DPB1*13:01:01G and HLA-A *11:01~C*07:02~B*38:02~DRB1*15:02~DQB1*05:01~DPB1*05:01:01G in the family 2 were recombined between HLA-DQB1 and HLA-DPB1 loci, which formed the haplotype of HLA-A*02:06~C*03:03~B*35:01~ DRB1*08:02~DQB1*04:02~DPB1*05:01:01G.
CONCLUSION:The gene recombination events between HLA-B and -DRB1, HLA-DQB1 and -DPB1 loci were found respectively in two Chinese Han families.
