Expressions and Clinical Significance of Notch1 and Hes1 in Diffuse Large B-Cell Lymphoma.
10.19746/j.cnki.issn.1009-2137.2023.03.015
- Author:
Yan LIU
1
,
2
;
Sha-Sha XU
1
,
3
;
Xiao-Ning WANG
4
;
Na ZHENG
4
;
Meng-Yao XU
4
;
Jie LI
5
Author Information
1. Department of Postgraduate of Hebei North University, Zhangjiakou 075000, Hebei Province, China
2. Department of Hematology, Hebei General Hospital, Shijiazhuang 050000, Hebei Province, China.
3. Department 4, The Second Affiliated Hospital of Luohe Medical College, Luohe 462300, Henan Province, China.
4. Department of Hematology, Hebei General Hospital, Shijiazhuang 050000, Hebei Province, China.
5. Department of Hematology, Hebei General Hospital, Shijiazhuang 050000, Hebei Province, China, E-mail: doclijie@163.com.
- Publication Type:Journal Article
- Keywords:
Hes1;
Notch1;
clinical feature;
diffuse large B-cell lymphoma
- MeSH:
Humans;
Cell Line;
Clinical Relevance;
Lymphadenitis;
Lymphoma, Large B-Cell, Diffuse/pathology*;
Prognosis;
RNA, Messenger
- From:
Journal of Experimental Hematology
2023;31(3):714-721
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expressions of Notch1 and Hes1 in diffuse large B-cell lymphoma (DLBCL), and their correlations with clinical features.
METHODS:Immunohistochemistry (IHC) was performed on DLBCL samples (54 cases) and lymphadenitis tissues (20 cases) to evaluate the expressions of Notch1 and Hes1, and analyze their correlations with clinical characteristics of patients. Based on Oncomine database, the expressions of Notch1 and Hes1 mRNA and DNA were also explored.
RESULTS:IHC result showed that the positive expression rates of Notch1 and Hes1 in DLBCL patients were significantly higher than those in the control group (P <0.05). In DLBCL patients, the expression of Notch1 was closely associated with B symptoms, Ann Arbor stage, lymphocyte count and the level of lactate dehydrogenase (P <0.05), while the expression level of Hes1 was significantly higher in patients with B symptoms (P <0.05). Notch+/Hes1+ expression was found in 21 DLBCL tissues (38.9%), and there was a correlation between Notch1 and Hes1 expression (r =0.296, P <0.05). Bioinformatics analysis (Oncomine database) showed that the mRNA expressions of Notch1 and Hes1 in the Brune dataset were significantly higher than those in the control tissues (P <0.05).
CONCLUSION:The expressions of Notch1 and Hes1 in DLBCL are significantly higher than those in lymphadenitis, and correlated with B symptoms and Ann Arbor stage, suggesting that Notch1 and Hes1 play important roles in the occurrence and development of DLBCL.
