Expression and Clinical Significance of Exosome Derived MiR-181b-5p in Children with Acute Lymphoblastic Leukemia.
10.19746/j.cnki.issn.1009-2137.2023.03.004
- Author:
Yi HONG
1
;
Kang-Kang LIU
1
;
Ning-Ling WANG
2
;
Zhi-Wei XIE
1
;
Jin-Hua CHU
1
Author Information
1. Department of Pediatrics, The Second Hospital of Anhui Medical University, Hefei 230061, Anhui Province, China.
2. Department of Pediatrics, The Second Hospital of Anhui Medical University, Hefei 230061, Anhui Province, China,E-mail:zwnltt@126.com.
- Publication Type:Journal Article
- Keywords:
acute lymphoblastic leukemia;
children;
clinical characteristics;
exosomes;
miR-181b-5p
- MeSH:
Humans;
Male;
Female;
Child;
Exosomes/metabolism*;
Clinical Relevance;
MicroRNAs/genetics*;
Precursor Cell Lymphoblastic Leukemia-Lymphoma/metabolism*;
Tumor Microenvironment
- From:
Journal of Experimental Hematology
2023;31(3):643-648
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the expression level of exosome derived miR-181b-5p in different disease stages of children with acute lymphoblastic leukemia and its relationship with clinical characteristics.
METHODS:Bone marrow plasma samples of 86 children with ALL were collected. Exosomes were extracted by exosome extraction kit, and RNA in exosomes was extracted by TRIzol method. The levels of miR-181b-5p in the blood plasma exosomes of the patients in the newly diagnosed group, relapse group, remission group and control group were detected by qRT- PCR. The difference of miR-181b-5p expression level in each group was compared and analyzed, and the relationship between miR-181b-5p expression level and clinical characteristics was analyzed.
RESULTS:The expression level of exosomal miR-181b-5p in the newly diagnosed group and the relapsed group was significantly lower than that in the remission group and the control group (P< 0.05). The expression level of exosomal miR-181b-5p in T-ALL children was higher than that in B-ALL children (P<0.05). The expression level of plasma exosomal miR-181b-5p in male children was higher than that in female children (P<0.01).
CONCLUSION:Exosome derived miR-181b-5p changes dynamically in the course of ALL children, and can be used as a marker miRNA to monitor disease status. Exosomes can transmit information in the tumor microenvironment and serve as a potential carrier for biomolecular targeted therapy.
