The Distribution and Significance of Activated T Cells and Lymphocyte Subsets in Myelodysplastic Syndrome.
10.19746/j.cnki.issn.1009-2137.2023.02.023
- Author:
Zhong-Li HU
1
;
Yan-Li YANG
2
;
Ping-Ping ZHANG
1
;
Shao-Jun PAN
3
Author Information
1. Department of Hematology, The First Affilicated Hospital of Bengbu Medical College, Bengbu 233000, Anhui Province, China.
2. Department of Hematology, The First Affilicated Hospital of Bengbu Medical College, Bengbu 233000, Anhui Province, China,E-mail：13855249678@139.com.
3. School of Translational Medicine, Shanghai Jiaotong University, Shanghai 201100, China.
- Publication Type:Journal Article
- Keywords:
WT1;
activated T cells;
lymphocyte subsets;
myelodysplastic syndrome
- MeSH:
Humans;
Lymphocyte Subsets;
Myelodysplastic Syndromes/diagnosis*;
Bone Marrow;
B-Lymphocytes;
Killer Cells, Natural;
Flow Cytometry;
T-Lymphocyte Subsets
- From:
Journal of Experimental Hematology
2023;31(2):469-475
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the distribution of bone marrow lymphocyte subsets in patients with myelodysplastic syndrome(MDS),the proportion of activated T cells with immunophenotype CD3+HLA-DR+ in the lymphocytes and its clinical significance, and to understand the effects of different types of MDS, different immunophenotypes, and different expression levels of WT1 on the proportion of lymphocyte subsets and activated T cells.
METHODS:The immunophenotypes of 96 MDS patients, the subsets of bone marrow lymphocytes and activated T cells were detected by flow cytometry. The relative expression of WT1 was detected by real-time fluorescent quantitative PCR, and the first induced remission rate (CR1) was calculated, the differences of lymphocyte subsets and activated T cells in MDS patients with different immunophenotype, different WT1 expression, and different course of disease were analyzed.
RESULTS:The percentage of CD4+T lymphocyte in MDS-EB-2, IPSS high-risk, CD34+ cells >10%, and patients with CD34+CD7+ cell population and WT1 gene overexpression at intial diagnosis decreased significantly (P<0.05), and the percentage of NK cells and activated T cells increased significantly (P<0.05), but there was no significant difference in the ratio of B lymphocytes. Compared with the normal control group, the percentage of NK cells and activated T cells in IPSS-intermediate-2 group was significantly higher(P<0.05), but there was no significant difference in the percentage of CD3+T, CD4+T lymphocytes. The percentage of CD4+T cells in patients with complete remission after the first chemotherapy was significantly higher than in patients with incomplete remission(P<0.05), and the percentage of NK cells and activated T cells was significantly lower than that in patients with incomplete remission (P<0.05).
CONCLUSION:In MDS patients, the proportion of CD3+T and CD4+T lymphocytes decreased, and the proportion of activated T cells increased, indicating that the differentiation type of MDS is more primitive and the prognosis is worse.
