Effect of P-coumaric Acid on Apoptosis of Multiple Myeloma Cells Based on Oxidative Stress.
10.19746/j.cnki.issn.1009-2137.2023.02.018
- Author:
Zhu-Fa HOU
1
;
Bing-Jie ZHAO
2
;
Song-Shan LIU
3
;
Wen-Jing YI
1
;
Hong CHE
1
Author Information
1. Department of Hematology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610072, Sichuan Province, China.
2. Department of Hematology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610072, Sichuan Province, China .E-mail: zhaobingjiedk @163.com.
3. Department of Hematology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610072, Sichuan Province, China . E-mail: pine009@163.com.
- Publication Type:Journal Article
- Keywords:
multiple myeloma;
Oxidative stress;
P-coumaric acid;
cell apoptosis
- MeSH:
Humans;
Reactive Oxygen Species/pharmacology*;
Cell Line, Tumor;
Multiple Myeloma;
Oxidative Stress;
Apoptosis
- From:
Journal of Experimental Hematology
2023;31(2):435-441
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of p-coumaric acid on apoptosis of multiple myeloma cells and its related mechanism.
METHODS:Multiple myeloma cell line MM.1s cells were selected and treated with different concentrations of p-coumaric acid (0, 0.4, 0.8, 1.6, 3.2 mmol/L), and the inhibition rate and half inhibition concentration (IC50) were detected by CCK-8 method. Then MM.1s cells were treated with 1/2 IC50, IC50, 2 IC50 and transfected with ov-Nrf-2 and ov-Nrf-2+IC50. The apoptosis, ROS fluorescence intensity and mitochondrial membrane potential of MM.1s cells were detected by flow cytometry, and the relative expressions of cellular Nrf-2 and HO-1 protein were detected by Western blot.
RESULTS:P-coumaric acid inhibited the proliferation of MM.1s cells in a dose-dependent manner(r =0.997) with an IC50 value of 2.754 mmol/L. Compared with the control group, apoptosis and ROS fluorescence intensity of MM.1s cells were significantly increased in the 1/2 IC50 group, IC50 group, 2 IC50 group and ov-Nrf-2+IC50 group (P <0.01), the expressions of Nrf-2, HO-1 protein in the IC50 group and 2 IC50 group were significantly decreased (P <0.05). Compared with the IC50 group, the cells apoptosis and ROS fluorescence intensity were significantly decreased (P <0.01), and the expressions of Nrf-2 and HO-1 protein were significantly increased in the ov-Nrf-2+IC50 group (P <0.01).
CONCLUSION:P-coumaric acid can inhibit the proliferation of MM.1s cells and may target the Nrf-2/HO-1 signaling pathway to affect oxidative stress in MM cells thereby inducing their apoptosis.
