Inhibitory Effect of Kaempferol on Proliferation of KG1a Cells and Its Mechanism.
10.19746/j.cnki.issn.1009-2137.2023.02.002
- Author:
Zhe CHEN
1
;
Ling ZHANG
1
;
Xiao-Fei YUAN
1
;
Bing-Hua GAO
2
;
Bin ZHANG
3
;
Xia WANG
3
Author Information
1. Department of Hematology,The First Affiliated Hospital of Hebei North University, Zhangjiakou 075061, Hebei Province, China.
2. Department of Hematology,The First Affiliated Hospital of Hebei North University, Zhangjiakou 075061, Hebei Province, China. E-mail: gaobinghua321@163.com.
3. Department of Clinical Laboratory Examination, The First Affiliated Hospital of Hebei North University, Zhangjiakou 075061, Hebei Province, China.
- Publication Type:Journal Article
- Keywords:
Janus kinase 2/signal transducer and activator of transcription 3;
acute myeloid leukemia;
apoptosis;
kaempferol;
proliferation
- MeSH:
Humans;
STAT3 Transcription Factor/metabolism*;
Interleukin-6/metabolism*;
Kaempferols/pharmacology*;
Signal Transduction;
Apoptosis;
Janus Kinase 2;
Cell Proliferation;
Leukemia, Myeloid, Acute
- From:
Journal of Experimental Hematology
2023;31(2):319-326
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of kaempferol on proliferation of acute myeloid leukemia (AML) KG1a cells and its mechanism.
METHODS:Human AML KG1a cells in logarithmic growth stage were taken and set at 25, 50, 75 and 100 μg/ml kaempferol group, another normal control group (complete medium without drug) and solvent control group (add dimethyl sulfoxide) were also set. After 24 and 48 hours of intervention, the cell proliferation rate was detected by CCK-8 assay. In addition, interleukin-6 (IL-6) combined with kaempferol group (Plus 20 μg/l IL-6 and 75 μg/ml kaempferol) was set up, 48 hours after culture, the cell cycle and apoptosis of KG1a cells were detected by flow cytometry, the mitochondrial membrane potential (MMP) of KG1a cells was detected by MMP detection kit (JC-1 method), and the expression of Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) pathway related proteins in KG1a cells were detected by Western blot.
RESULTS:The cell proliferation rate of 25, 50, 75 and 100 μg/ml kaempferol group decreased significantly (P<0.05), and with the increase of kaempferol dose (r24 h=-0.990, r48 h= -0.999), the cell proliferation rate decreased gradually (P<0.05). The inhibitory effect of 75 μg/ml kaempferol on cell proliferation reached half of effective dose after 48 hours of intervention. Compared with normal control group, the G0/G1 phase cell proportion and apoptosis rate of cells in 25, 50 and 75 μg/ml kaempferol group increased, while the S phase cell proportion, MMP, phosphorylated JAK2 (p-JAK2)/JAK2 and phosphorylated STAT3 (p-STAT3)/STAT3 protein expression decreased in a dose-dependent manner (r=0.998, 0.994, -0.996, -0.981, -0.997, -0.930). Compared with 75 μg/ml kaempferol group, the G0/G1 phase cell proportion and apoptosis rate of cells in IL-6 combined with kaempferol group decreased, while the S phase cell proportion, MMP, p-JAK2/JAK2 and p-STAT3/STAT3 protein expression increased significantly (P<0.05).
CONCLUSION:Kaempferol can inhibit KG1a cell proliferation and induce KG1a cell apoptosis, its mechanism may be related to the inhibition of JAK2/STAT3 signal pathway.
