Establishment of a Patient-Derived T-Cell Acute Lymphoblastic Leukemia Xenograft Model in Novel Immunodeficient NCG Mice.
10.19746/j.cnki.issn.1009-2137.2023.02.001
- Author:
Peng-Jun JIANG
1
;
Xing-Bin DAI
1
;
Xiang-Tu KONG
1
;
Zu-Qiong XU
1
;
Hui YU
1
;
Jie PANG
1
;
Wen XIA
1
;
Ju-Hua YU
1
;
Guang-Rong ZHU
1
;
Fang TIAN
2
;
Xue-Jun ZHU
3
Author Information
1. Department of Hematology, The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing 210029, Jiangsu Province, China.
2. Central Laboratory, The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing 210029, Jiangsu Province, China .E-mail: tfgzyxh1986@njucm.edu.cn.
3. Department of Hematology, The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing 210029, Jiangsu Province, China.E-mail: zhuxj2@sina.com.
- Publication Type:Journal Article
- Keywords:
NCG mice;
T-cell acute lymphoblastic leukemia;
animal model;
immunodeficiency;
xenograft
- MeSH:
Humans;
Animals;
Mice;
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma;
Heterografts;
Bone Marrow;
Disease Models, Animal;
T-Lymphocytes;
Mice, SCID
- From:
Journal of Experimental Hematology
2023;31(2):311-318
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:The leukemia cells from patients with T-cell acute lymphoblastic leukemia (T-ALL) were inoculated into NCG mice to establish a stable human T-ALL leukemia animal model.
METHODS:Leukemia cells from bone marrow of newly diagnosed T-ALL patients were isolated, and the leukemia cells were inoculated into NCG mice via tail vein. The proportion of hCD45 positive cells in peripheral blood of the mice was detected regularly by flow cytometry, and the infiltration of leukemia cells in bone marrow, liver, spleen and other organs of the mice was detected by pathology and immunohistochemistry. After the first generation mice model was successfully established, the spleen cells from the first generation mice were inoculated into the second generation mice, and after the second generation mice model was successfully established, the spleen cells from the second generation mice were further inoculated into the third generation mice, and the growth of leukemia cells in peripheral blood of the mice in each group was monitored by regular flow cytometry to evaluate the stability of this T-ALL leukemia animal model.
RESULTS:On the 10th day after inoculation, hCD45+ leukemia cells could be successfully detected in the peripheral blood of the first generation mice, and the proportion of these cells was gradually increased. On average, the mice appeared listless 6 or 7 weeks after inoculation, and a large number of T lymphocyte leukemia cells were found in the peripheral blood and bone marrow smear of the mice. The spleen of the mice was obviously enlarged, and immunohistochemical examination showed that hCD3+ leukemia cells infiltrated into bone marrow, liver and spleen extensively. The second and third generation mice could stably develop leukemia, and the average survival time was 4-5 weeks.
CONCLUSION:Inoculating leukemia cells from bone marrow of patients with T-ALL into NCG mice via tail vein can successfully construct a patient-derived tumor xenografts (PDTX) model.
