Protective effect of melatonin against oxygen-induced retinopathy: a study based on the HMGB1/NF-κB/NLRP3 axis.
10.7499/j.issn.1008-8830.2301036
- Author:
Fang-Fang CHU
1
,
2
;
Yan-Song ZHAO
;
Yu-Ze ZHAO
;
Chen BAI
1
,
2
;
Pei-Lun XIAO
1
,
2
;
Xiao-Li WANG
;
Shu-Na YU
1
,
2
;
Ji-Ying JIANG
1
,
2
Author Information
1. Department of Anatomy, Weifang Medical College, Weifang, Shandong 261053, China
2. Ophthalmology Center, Affiliated Hospital of Weifang Medical College, Weifang, Shandong 261031, China.
- Publication Type:Journal Article
- Keywords:
HMGB1/NF-κB/NLRP3 axis;
Inflammation;
Melatonin;
Neonatal mouse;
Oxygen-induced retinopathy
- MeSH:
Animals;
Mice;
HMGB1 Protein;
Melatonin/therapeutic use*;
Mice, Inbred C57BL;
NF-kappa B;
NLR Family, Pyrin Domain-Containing 3 Protein;
Oxygen/adverse effects*;
Peroxidase;
Receptors, Melatonin;
Retinal Diseases/drug therapy*
- From:
Chinese Journal of Contemporary Pediatrics
2023;25(6):645-652
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To study the protective effect of melatonin (Mel) against oxygen-induced retinopathy (OIR) in neonatal mice and the role of the HMGB1/NF-κB/NLRP3 axis.
METHODS:Neonatal C57BL/6J mice, aged 7 days, were randomly divided into a control group, a model group (OIR group), and a Mel treatment group (OIR+Mel group), with 9 mice in each group. The hyperoxia induction method was used to establish a model of OIR. Hematoxylin and eosin staining and retinal flat-mount preparation were used to observe retinal structure and neovascularization. Immunofluorescent staining was used to measure the expression of proteins and inflammatory factors associated with the HMGB1/NF-κB/NLRP3 axis and lymphocyte antigen 6G. Colorimetry was used to measure the activity of myeloperoxidase.
RESULTS:The OIR group had destruction of retinal structure with a large perfusion-free area and neovascularization, while the OIR+Mel group had improvement in destruction of retinal structure with reductions in neovascularization and perfusion-free area. Compared with the control group, the OIR group had significant increases in the expression of proteins and inflammatory factors associated with the HMGB1/NF-κB/NLRP3 axis, the expression of lymphocyte antigen 6G, and the activity of myeloperoxidase (P<0.05). Compared with the OIR group, the OIR+Mel group had significant reductions in the above indices (P<0.05). Compared with the control group, the OIR group had significant reductions in the expression of melatonin receptors in the retina (P<0.05). Compared with the OIR group, the OIR+Mel group had significant increases in the expression of melatonin receptors (P<0.05).
CONCLUSIONS:Mel can alleviate OIR-induced retinal damage in neonatal mice by inhibiting the HMGB1/NF-κB/NLRP3 axis and may exert an effect through the melatonin receptor pathway.
