Effect of procalcitonin on lipopolysaccharide-induced expression of nucleotide-binding oligomerization domain-like receptor protein 3 and caspase-1 in human umbilical vein endothelial cells.
10.7499/j.issn.1008-8830.2301031
- Author:
Wen JIANG
1
;
Ding-Hua SHI
1
;
Yan-Juan HE
1
;
Chun-Yuan CHEN
1
Author Information
1. Department of Pediatrics, Third Xiangya Hospital, Central South University, Changsha 410013, China.
- Publication Type:Journal Article
- Keywords:
Caspase-1;
Human umbilical vein endothelial cell;
Nucleotide-binding oligomerization domain-like receptor protein 3;
Procalcitonin;
Pyroptosis;
Sepsis
- MeSH:
Humans;
Caspase 1/metabolism*;
Human Umbilical Vein Endothelial Cells/metabolism*;
Lipopolysaccharides/pharmacology*;
NLR Family, Pyrin Domain-Containing 3 Protein/metabolism*;
Procalcitonin;
Nucleotides/pharmacology*
- From:
Chinese Journal of Contemporary Pediatrics
2023;25(5):521-526
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To study the effect of procalcitonin (PCT) on lipopolysaccharide (LPS)-induced expression of the pyroptosis-related proteins nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) and caspase-1 in human umbilical vein endothelial cells (HUVECs).
METHODS:HUVECs were induced by LPS to establish a model of sepsis-induced inflammatory endothelial cell injury. The experiment was divided into two parts. In the first part, HUVECs were randomly divided into four groups: normal control, LPS (1 μg/mL), PCT (10 ng/mL), and LPS+PCT (n=3 each). In the second part, HUVECs were randomly grouped: normal control, LPS, and LPS+PCT of different concentrations (0.1, 1, 10, and 100 ng/mL) (n=3 each). Quantitative real-time PCR and Western blot were used to measure the mRNA and protein expression levels of NLRP3 and caspase-1 in each group.
RESULTS:In the first experiment: compared with the normal control group, the PCT, LPS, and LPS+PCT groups had significantly upregulated mRNA and protein expression levels of NLRP3 and caspase-1 (P<0.05); compared with the LPS group, the LPS+PCT group had significantly downregulated mRNA and protein expression levels of NLRP3 and caspase-1 (P<0.05). In the second experiment: compared with those in the LPS group, the mRNA and protein expression levels of NLRP3 and caspase-1 in the LPS+PCT of different concentrations groups were significantly downregulated in a concentration-dependent manner (P<0.05).
CONCLUSIONS:LPS can promote the expression of the pyroptosis-related proteins NLRP3 and caspase-1 in HUVECs, while PCT can inhibit the LPS-induced expression of the pyroptosis-related proteins NLRP3 and caspase-1 in HUVECs in a concentration-dependent manner.
