The expression of autophagy-related proteins in peripheral blood mononuclear cells of patients with lupus nephritis is upregulated and related to kidney damage.
- Author:
Weiguang WANG
1
;
Kai ZHANG
2
;
Xiaopeng SUN
3
;
Bingbing FU
4
Author Information
1. Department of Hematopathology, The First Affiliated Hospital of Jiamusi University, Jiamusi 154002, China.
2. Department of Rheumatology, Affiliated Hospital of Jining Medical University, Jining 272029, China.
3. Department of Rheumatology, The First Affiliated Hospital of Jiamusi University, Jiamusi 154002, China.
4. Department of Rheumatology, The First Affiliated Hospital of Jiamusi University, Jiamusi 154002, China. *Corresponding author, E-mail: fubingbingfbb@163.com.
- Publication Type:Journal Article
- MeSH:
Humans;
Tumor Necrosis Factor-alpha/metabolism*;
Leukocytes, Mononuclear/metabolism*;
Autophagy-Related Proteins/metabolism*;
Lupus Nephritis/urine*;
Kidney;
Interferon-gamma/metabolism*;
Inflammation/metabolism*;
Lupus Erythematosus, Systemic/metabolism*
- From:
Chinese Journal of Cellular and Molecular Immunology
2023;39(7):633-637
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the relationship between nephritis activity, autophagy and inflammation in patients with SLE. Methods Western blot analysis was used to detect the expression of microtubule-associated protein 1 light chain 3 (LC3) and P62 in peripheral blood mononuclear cells (PBMCs) of SLE patients with lupus nephritis and non-lupus nephritis patients. Tumor necrosis factor α (TNF-α) and interferon γ (IFN-γ) in the serum of SLE patients were determined by ELISA. The correlation between LC3II/LC3I ratio and SLE disease activity score (SLEDAI), urinary protein, TNF-α and IFN-γ levels was analyzed by Pearson method. Results The expression of LC3 was increased and P62 was decreased in SLE patients. TNF-α and IFN-γ were increased in the serum of SLE patients. LC3II/LC3I ratio was positively correlated with SLEDAI (r=0.4560), 24 hour urine protein (r=0.3753), IFN-γ (r=0.5685), but had no correlation with TNF-α (r=0.04 683). Conclusion Autophagy is found in PBMCs of SLE, and the autophagy is correlated with renal damage and inflammation in patients with lupus nephritis.
