LPS-induced endothelial cytoskeleton remodeling in human lung vessels and related miRNAs-profiling.
- Author:
Yuzhen LYU
1
,
2
,
3
;
Wenqin YU
1
,
2
,
3
;
Yulu YANG
4
;
Xiaolan XUE
4
;
Haibin MA
5
;
Xiaowei MA
6
Author Information
1. Clinical Medical College, Ningxia Medical University, Yinchuan 750004
2. Human Stem Cell Institute, General Hospital of Ningxia Medical University, Yinchuan 750004
3. Intensive Care Unit, Cardiocerebral Vascular Disease Hospital, General Hospital of Ningxia Medical University, Yinchuan 750012, China.
4. Clinical Medical College, Ningxia Medical University, Yinchuan 750004, China.
5. Human Stem Cell Institute, General Hospital of Ningxia Medical University, Yinchuan 750004, China.
6. Intensive Care Unit, Cardiocerebral Vascular Disease Hospital, General Hospital of Ningxia Medical University, Yinchuan 750012, China. *Corresponding author, E-mail: mxwei-99@ 163.com.
- Publication Type:Journal Article
- MeSH:
Humans;
Lipopolysaccharides/pharmacology*;
Endothelial Cells/metabolism*;
MicroRNAs/metabolism*;
Lung/metabolism*;
Cytoskeleton;
Gene Expression Profiling
- From:
Chinese Journal of Cellular and Molecular Immunology
2023;39(7):592-598
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of lipopolysaccharide (LPS) on human pulmonary vascular endothelial cells (HPVECs) cytoskeleton and perform biological analysis of the microRNA (miRNA) spectrum. Methods The morphology of HPVECs was observed by microscope, the cytoskeleton by FITC-phalloidin staining, and the expression of VE-cadherin was detected by immunofluorescence cytochemical staining; the tube formation assay was conducted to examine the angiogenesis, along with cell migration test to detect the migration, and JC-1 mitochondrial membrane potential to detect the apoptosis. Illumina small-RNA sequencing was used to identify differentially expressed miRNAs in NC and LPS group. The target genes of differentially expressed miRNAs were predicted by miRanda and TargetScan, and the functional and pathway enrichment analysis was performed on Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Further biological analysis of related miRNAs was carried out. Results After the LPS got induced, the cells became round and the integrity of cytoskeleton was destroyed. The decreased expression of VE-cadherin was also observed, along with the decreased ability of angiogenesis and migration, and increased apoptosis. Sequencing results showed a total of 229 differential miRNAs, of which 84 miRNA were up-regulated and 145 miRNA were down-regulated. The target gene prediction and functional enrichment analysis of these differential miRNA showed that they were mainly concentrated in pathways related to cell connection and cytoskeleton regulation, cell adhesion process and inflammation. Conclusion In vitro model of lung injury, multiple miRNAs are involved in the process of HPVECs cytoskeleton remodeling, the reduction of barrier function, angiogenesis, migration and apoptosis.
