Knock-down of long intergenic noncoding RNA cyclooxygenase 2 (lincRNA-COX2) inhibits apoptosis and polarization into M1 in Listeria monocytogenes-infected macrophages.
- Author:
Yurong ZHU
1
,
2
;
Shuang HUANG
3
;
Lin LIN
3
;
Fengyuan ZHANG
3
;
Xugan JIANG
3
;
Shengxia CHEN
4
Author Information
1. School of Medicine, Jiangsu University, Zhenjiang 212013
2. Clinical Microbiology, Linfen Central Hospital, Linfen 041000, China.
3. School of Medicine, Jiangsu University, Zhenjiang 212013, China.
4. School of Medicine, Jiangsu University, Zhenjiang 212013, China. *Corresponding author, E-mail: chensxia@ujs.edu.cn.
- Publication Type:Journal Article
- MeSH:
Apoptosis/genetics*;
bcl-2-Associated X Protein/metabolism*;
Caspase 3/metabolism*;
Cyclooxygenase 2/metabolism*;
Listeria monocytogenes/pathogenicity*;
Macrophages/microbiology*;
RNA, Long Noncoding/metabolism*;
RNA, Small Interfering/genetics*;
Animals;
Mice
- From:
Chinese Journal of Cellular and Molecular Immunology
2023;39(4):289-294
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of long intergenic non-coding RNA COX2 (lincRNA-COX2) on apoptosis and polarization of Listeria monocytogenes (Lm)-infected RAW264.7 cells. Methods RAW264.7 cells were cultured and divided into control group (uninfected cells), Lm infection group, negative control of small interfering RNA (si-NC) group, si-NC and Lm infection group, small interfering RNA of lincRNA-COX2 (si-lincRNA-COX2) group, si-lincRNA-COX2 and Lm infection group. RAW264.7 cells were infected with MOI=10 Lm for 6 hours, and then the inhibition efficiency of siRNA transfection was detected by fluorescence microscope and quantitative real-time PCR (qRT-PCR). The expression levels of cleaved-caspase-3(c-caspase-3), caspase-3, B-cell lymphoma-2 (Bcl2), Bcl2 associated X protein (BAX), arginase 1 (Arg1), inducible nitric oxide synthase (iNOS) were detected by Western blot analysis. Results c-caspase-3/caspase-3, BAX/Bcl2 and iNOS were significantly up-regulated, while the level of Arg1 was down-regulated in Lm-infected RAW264.7 cells compared with control group. LincRNA-COX2 knockdown inhibited the increase of protein levels for BAX/Bcl2, c-caspase-3/caspase-3 and iNOS in Lm-infected RAW264.7 cells, while the level of Arg1 in Lm-infected RAW264.7 cells was up-regulated. Conclusion Knockdown of lincRNA-COX2 can inhibit cell apoptosis and suppress the macrophage polarization into M1 type in Lm-infected RAW264.7 cells.
