Analysis of Forty-Two Psychoactive Substances in a Single Hair by Micro-Segmental Technique.
10.12116/j.issn.1004-5619.2022.321101
- Author:
Jiao-Jiao JI
1
;
Duo-Qi XU
1
;
Ping XIANG
1
;
Hui YAN
1
;
Min SHEN
1
Author Information
1. Shanghai Key Laboratory of Forensic Medicine, Key Laboratory of Forensic Science, Ministry of Justice, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China.
- Publication Type:Journal Article
- Keywords:
drug-facilitated sexual assault;
forensic medicine;
liquid chromatography-tandem mass spectrometry (LC-MS/MS);
micro-segmentation;
psychoactive substance;
single hair;
toxicological analysis;
zolpidem
- MeSH:
Humans;
Chromatography, Liquid/methods*;
Zolpidem;
Tandem Mass Spectrometry/methods*;
Hair;
Acetonitriles;
Chromatography, High Pressure Liquid
- From:
Journal of Forensic Medicine
2023;39(2):151-160
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To establish an LC-MS/MS method based on single hair micro-segmental technique, and verify the detection of 42 psychoactive substances in 0.4 mm hair segments.
METHODS:Each piece of single hair was cut into 0.4 mm segments and extracted by sonication and the segments were immersed in dithiothreitol-containing extraction medium. Mobile phase A was the aqueous solution containing 20 mmol/L ammonium acetate, 0.1% formic acid, and 5% acetonitrile. Mobile phase B was acetonitrile. An electrospray ionization source in positive ion mode was used for data acquisition in multiple reaction monitoring (MRM) mode.
RESULTS:The 42 psychoactive substances in hair had a good linear relationship within their respective linear ranges (r>0.99), the limits of detection were 0.2-10 pg/mm, the limits of quantification were 0.5-20 pg/mm, the intra-day and inter-day precisions were 1.5%-12.7%, the intra-day and inter-day accuracies were 86.5%-109.2%, the recovery rates were 68.1%-98.2%, and the matrix effects were 71.3%-111.7%. The method was applied to hair samples collected from one volunteer at 28 d after a single dose of zolpidem, with zolpidem detected in 5 hairs was 1.08-1.60 cm near the root tip, and the concentration range was 0.62-20.5 pg/mm.
CONCLUSIONS:The micro-segmental technique of single hair analysis can be applied to the investigation of drug-facilitated sexual assault cases.
