Evaluation of HiSense(TM) Reagents for Chemistry Autoanalyzer.
- Author:
Hee Yeon WOO
1
;
Young Jae KIM
;
Hyosoon PARK
Author Information
1. Department of Laboratory Medicine, Sungkyunkwan University School of Medicine Kangbuk Samsung Hospital, Seoul, Korea. hspcp@naver.com
- Publication Type:Original Article
- Keywords:
HiSense reagents;
Precision;
Linearity;
Correlation;
Interference
- MeSH:
Alanine Transaminase;
Alkaline Phosphatase;
Aspartate Aminotransferases;
Bilirubin;
Blood Glucose;
Calcium;
Chemistry*;
Cholesterol;
Creatinine;
Indicators and Reagents*;
L-Lactate Dehydrogenase;
Nitrogen;
Phosphorus;
Transferases;
Triglycerides;
Urea;
Uric Acid
- From:Journal of Laboratory Medicine and Quality Assurance
2006;28(1):189-198
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: As many kinds of reagents for chemistry autoanalyzer have been developed, comprehensive evaluation of the reagents is needed for proper selection. We evaluated the analytical performances of the HiSense(TM) reagents (HBI Co., Ltd, An-yang, Korea). METHODS: Sixteen chemistry reagents of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma glutamyl transferase (GGT), total protein, total bilirubin, albumin, glucose, blood urea nitrogen (BUN), creatinine, total calcium, phosphorus, lactate dehydrogenase (LDH), uric acid, total cholesterol, and triglyceride (TG) were evaluated for linearity, precision, interference, and correlation according to CLSI guidelines in the Toshiba ACCURATE chemistry autoanalyzer (Toshiba Medical Systems Co., Ltd., Tokyo, Japan). RESULTS: The coefficients of variation (CV) of both within-run and total precision were below 2.6% for all analytes. Good linearity was observed for all analytes in measurement ranges. Coefficients of correlation were also good for all analytes. There were clinically significant effects of interfering factors, hemoglobin and lipid, in LDH and total protein, respectively. CONCLUSIONS: HiSense reagents showed good precision, linearity, and correlation. We conclude that these reagents are appropriate for routine clinical use.
