Progress on three-dimensional cell culture technology and their application.
10.7507/1001-5515.202204062
- Author:
Xiaoqin LU
1
;
Xiaofeng LIU
1
;
Hao ZHONG
1
;
Wei ZHANG
1
;
Shuzhen YU
1
;
Rongfa GUAN
1
Author Information
1. College of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, P. R. China.
- Publication Type:Journal Article
- Keywords:
Active substance;
Application;
Functional evaluation;
In vitro culture;
Three-dimensional cell culture
- MeSH:
Cell Culture Techniques, Three Dimensional;
Cell Culture Techniques;
Apoptosis;
Cell Proliferation;
Technology
- From:
Journal of Biomedical Engineering
2023;40(3):602-608
- CountryChina
- Language:Chinese
-
Abstract:
Three-dimensional (3D) cell culture model is a system that co-culture carriers with 3D structural materials and different types of cells in vitro to simulate the microenvironment in vivo. This novel cell culture model has been proved to be close to the natural system in vivo. In the process of cell attachment, migration, mitosis and apoptosis, it could produce biological reactions different from that of monolayer cell culture. Therefore, it can be used as an ideal model to evaluate the dynamic pharmacological effects of active substances and the metastasis process of cancer cells. This paper compared and analyzed the different characteristics of cell growth and development under two-dimensional (2D) and 3D model culture and introduced the establishment method of 3D cell model. The application progress of 3D cell culture technology in tumor model and intestinal absorption model was summarized. Finally, the application prospect of 3D cell model in the evaluation and screening of active substance was revealed. This review is expected to provide reference for the development and application of new 3D cell culture models.
