Effect of guarana on lipid metabolism in obese rats: a study based on lipidomics.
10.19540/j.cnki.cjcmm.20230224.401
- Author:
Min-Yan LIANG
1
;
Song WANG
1
;
Zi-Wei XU
1
;
Jing-Jing WANG
1
;
Xiao-Die HU
2
;
Hong-Ning LIU
3
;
Li-Ping HUANG
4
Author Information
1. School of Pharmacy, Jiangxi University of Chinese Medicine Nanchang 330004, China.
2. School of Pharmacy, Jiangxi University of Chinese Medicine Nanchang 330004, China College of Pharmacy, Jinan University Guangzhou 511436, China.
3. Advanced Research Institute, Jiangxi University of Chinese Medicine Nanchang 330004, China Jiangxi Province Key Laboratory of Chinese Medicine Etiopathogenisis Nanchang 330004, China.
4. School of Pharmacy, Jiangxi University of Chinese Medicine Nanchang 330004, China Jiangxi Provincial Key Laboratory of Traditional Chinese Medicine Pharmacology Nanchang 330004, China.
- Publication Type:Journal Article
- Keywords:
dyslipidemia;
guarana;
lipidomics;
obesity
- From:
China Journal of Chinese Materia Medica
2023;48(14):3865-3873
- CountryChina
- Language:Chinese
-
Abstract:
This study investigated the effect of guarana on plasma lipid metabolites in obese rats and analyzed its mechanism in the treatment of dyslipidemia in obesity. High-fat diet was used to establish obese rat models, and the therapeutic effect of guarana on obese rats was evaluated by measuring body weight, white fat, liver weight, and lipid content, as well as observing liver histomorphology. Lipid metabolites in plasma of rats in each group were detected by UHPLC-Q-TOF-MS lipidomics. The protein expressions of fatty acid synthase, acetyl-CoA carboxylase 1, triglyceride synthesis enzyme, carnitine palmitoyltransferase Ⅰ, and acetyl-coenzyme A acyltransferase 2 in rat liver were detected using Western blot. The results revealed that guarana significantly reduced body weight, white fat, and liver weight of obese rats due to high-fat diet, and alleviated dyslipidemia and liver steatosis. Lipidomics showed that some triglycerides and phospholipids were significantly elevated in the high-fat model group, and part of them was reduced after guarana treatment. Western blot found that guarana inhibited the expression of hepatic fatty acid and triglyceride synthesis-related proteins and increased the expression of fatty acid β-oxidation-related proteins. Abnormalities in triglyceride and phospholipid metabolism are the main characteristics of plasma lipid metabolism in obese rats induced by high-fat diet. Guarana may regulate partial triglyceride and phospholipid metabolism by inhibiting hepatic fatty acid and triglyceride synthesis and increasing fatty acid β-oxidation, thereby improving rat obesity and dyslipidemia.
