Chemical constituents from Callicarpa kwangtungensis and their hemostatic activities.
10.19540/j.cnki.cjcmm.20230411.201
- Author:
Shu-Tian XUE
1
;
Wei LIU
1
;
Tao YUAN
2
Author Information
1. Key Laboratory of Natural Product Chemistry and Application at Universities of Education Department of Xinjiang Uygur Autonomous Region, School of Chemistry and Environmental Science, Yili Normal University Yining 835000, China.
2. Key Laboratory of Natural Product Chemistry and Application at Universities of Education Department of Xinjiang Uygur Autonomous Region, School of Chemistry and Environmental Science, Yili Normal University Yining 835000, China College of Life Sciences, Jiangxi Normal University Nanchang 330022, China.
- Publication Type:Journal Article
- Keywords:
Callicarpa kwangtungensis;
chemical constituents;
hemostatic activity;
phenylethanoid glycosides
- MeSH:
Animals;
Mice;
Callicarpa;
Hemostatics;
China;
Glycosides/chemistry*
- From:
China Journal of Chinese Materia Medica
2023;48(14):3815-3825
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to identify the chemical constituents from Callicarpa kwangtungensis and determine their activities. MCI, ODS, and Sephadex LH-20 chromatography and semi-preparative HPLC were employed to separate the chemical constituents. A total of 15 compounds were separated, and their structures were identified on the basis of spectroscopic analysis and comparison with the data in relevant literature. Specifically, the 15 compounds were 3-O-α-L-rhamnopyranosyl-6-O-β-D-apiofuranosyl-4-O-E-caffeoyl-D-glucopyranoside(1), 3,6-O-α-L-dirhamnopyranosyl-4-O-E-caffeoyl-D-glucopyranoside(2), β-OH-forsythoside B(3), β-OH-poliumoside(4),(+)-lyoniresinol-3α-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranoside(5),(+)-lyoniresinol-3α-O-β-D-glucopyranoside(6),(-)-lyoniresinol-3α-O-β-D-glucopyranoside(7), kelampayoside A(8), descaffeoylpoliumoside(9), acteoside(10), alyssonoside(11), poliumoside(12), isacteoside(13), acetyl forsythoside B(14), and forsythoside B(15). Compounds 1 and 2 were novel, and the NMR data of compounds 3 and 4 were reported here for the first time. Furthermore, the hemostatic activities of the extract and abundant ingredients(compounds 12 and 15) of C. kwangtungensis were determined with Yunnan Baiyao as the positive control and normal saline as the negative control. The extract and compounds 12 and 15 significantly shortened the tail tip bleeding time in mice.
