Distinguishing between Artemisia stolonifera and A. argyi by specific PCR of leaves and non-glandular trichomes.
10.19540/j.cnki.cjcmm.20230409.101
- Author:
Ya-Chen ZHAO
1
;
Shuang-Ge LI
2
;
Hui LI
3
;
Yi-Mei LIU
2
;
Ting-Ting ZHAO
2
;
Yu-Huan MIAO
2
;
Da-Hui LIU
2
;
Lu-Qi HUANG
4
Author Information
1. Aademician Workstation, Jiangxi University of Chinese Medicine Nanchang 330004, China Institute of Traditional Chinese Medicine Health Industry, China Academy of Chinese Medical Sciences Nanchang 330000, China Jiangxi Health Industry Institute of Traditional Chinese Medicine Nanchang 330000, China.
2. Resource Center for Chinese Materia Medica, Hubei University of Chinese Medicine Wuhan 430065, China.
3. Institute of Traditional Chinese Medicine Health Industry, China Academy of Chinese Medical Sciences Nanchang 330000, China Jiangxi Health Industry Institute of Traditional Chinese Medicine Nanchang 330000, China.
4. China Academy of Chinese Medical Sciences Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
Artemisia argyi;
Artemisia stolonifera;
ITS2;
molecular identification;
non-glandular trichome;
single nucleotide polymorphism
- MeSH:
Artemisia/genetics*;
Trichomes;
Polymerase Chain Reaction;
Nucleic Acid Amplification Techniques;
Plant Leaves/genetics*
- From:
China Journal of Chinese Materia Medica
2023;48(14):3730-3735
- CountryChina
- Language:Chinese
-
Abstract:
Artemisia stolonifera is a relative of A. argyi. The two species are difficult to be distinguished due to the similarity in leaf shape and have even less distinctive features after processing. This study aims to establish a method to quickly distinguish between them. At the same time, we examined the reasonability and applicability of the specific polymerase chain reaction(PCR) method. The C/T single nucleotide polymorphism was detected at the position 202 of the sequence, based on which specific primers were designed to identify these two species. The PCR with the specific primer JNC-F and the universal primer ITS3R produced a specific band at 218 bp for A. argyi and no band for A. stolonifera, which can be used to detect at least 3% of A. argyi samples mixed in A. stolonifera samples. The PCR with the specific primer KY-F and the universal primer ITS3R produced a specific band at 218 bp for A. stolonifera and no band for A. argyi, which can be used to detect at least 5% of A. stolonifera samples mixed with A. argyi. The limit of detection of the established method was 5 ng DNA. The established PCR method can accurately distinguish between A. stolonifera and A. argyi, which provides an experimental basis for the quality control of A. stolonifera and determines whether the herbs are adulterated.
