Qualitative and quantitative study of flavonoids in Notoginseng Radix et Rhizoma based on UPLC-Q-TOF-MS and HPLC-DAD.
10.19540/j.cnki.cjcmm.20230227.101
- Author:
Jie YANG
1
;
Yan-Lin WANG
2
;
Di YANG
1
;
Xue DI
1
;
Xin-Tong HAN
1
;
Si-Yu LI
1
;
Fu-Gang WEI
2
;
Tian-Min WANG
1
Author Information
1. College of Pharmacy, Liaoning University of Traditional Chinese Medicine Dalian 116600, China.
2. Wenshan Miaoxiang Sanqi Technology Co., Ltd. Wenshan 663108, China.
- Publication Type:Journal Article
- Keywords:
HPLC-DAD;
Panax notoginseng;
UPLC-Q-TOF-MS;
anthocyanins;
flavonoids
- MeSH:
Flavonoids/analysis*;
Anthocyanins/analysis*;
Quercetin;
Chromatography, High Pressure Liquid/methods*;
Kaempferols;
Tandem Mass Spectrometry/methods*;
Glycosides
- From:
China Journal of Chinese Materia Medica
2023;48(13):3462-3471
- CountryChina
- Language:Chinese
-
Abstract:
The flavonoids in Panax notoginseng were qualitatively analyzed by ultra-high performance liquid chromatography-quadrupole-time of flight mass spectrometry(UPLC-Q-TOF-MS), and the content of three main flavonoids in P. notoginseng of different specifications and grades collected from different habitats was determined by HPLC-DAD. Flavonoids and anthocyanins were analyzed by UPLC-Q-TOF-MS/MS in the positive and negative ion modes, respectively. Twelve flavonoid glycosides and one anthocyanin glycoside in P. notoginseng were identified, but no flavonoid aglycones were detected. Among them, 12 compounds were identified in the underground part of P. notoginseng for the first time and eight compounds were first reported in this plant. Moreover, six and four compounds were identified in the Panax genus and the Araliaceae family for the first time, respectively. A method for simultaneous determination of three flavonoids in P. notoginseng was established by HPLC-DAD. The content of flavonoids in 721 P. notoginseng samples of 124 specifications and grades collected from 20 different habitats was simultaneously determined. Among three flavonoids determined, the content of quercetin-3-O-(2″-β-D-xylosyl)-β-D-galactoside was the highest with the average content in the tested samples of 161.0 μg·g~(-1). The content of compounds quercetin-3-O-hexosyl-hexoside and kaempferol-3-O-pentosyl-hexoside was relatively low, with the average content of 18.5 μg·g~(-1)(calculated as quercetin-3-O-sophoroside) and 49.4 μg·g~(-1)(calculated as kaempferol-3-O-sangbu diglycoside). There were significant differences in flavonoids content of samples from different production area. The content of flavonoids in spring P. notoginseng was significantly lower than that in winter P. notoginseng when the other influencing factors such as production areas, germplasm resources, and cultivation conditions were fixed. As for P. notoginseng of different specifications, the flavonoid content in the part connecting the taproot and the aboveground stem was significantly higher than that in other parts. The results of large-scale data showed that the flavonoid content gradually increased with the increase in the number of heads. There were significant differences between the flavonoid content in most specifications and grades, especially the 20-head P. notoginseng and countless head P. notoginseng, whose content was significantly lower and significantly higher than that of other specifications and grades, respectively. This study provides a scientific basis for the study of the effective components and quality control of P. notoginseng from the perspective of flavonoids.
