Difference in liver injury induced by dictamnine between males and females: based on untargeted metabolomics.
10.19540/j.cnki.cjcmm.20230119.401
- Author:
Can TU
1
;
Zhao-Juan GUO
1
;
Bing-Qian JIANG
1
;
Qian-Jun KANG
1
;
Ting WANG
1
Author Information
1. National Medical Products Administration Key Laboratory for Research and Evaluation of Traditional Chinese Medicine, Beijing International Science and Technology Cooperation Base for Traditional Chinese Medicine Hepatotoxicity and New Drug Research and Development, Beijing Research Institute of Chinese Medicine, Beijing University of Chinese Medicine Beijing 100029, China.
- Publication Type:Journal Article
- Keywords:
dictamnine;
difference between males and females;
liver injury;
mechanism;
untargeted metabolomics
- MeSH:
Female;
Male;
Animals;
Mice;
Tryptophan;
Metabolomics;
Fatty Liver;
Steroids;
Hormones
- From:
China Journal of Chinese Materia Medica
2023;48(12):3317-3326
- CountryChina
- Language:Chinese
-
Abstract:
In recent years, reports of adverse reactions related to traditional Chinese medicine(TCM) have been on the rise, especially some traditionally considered "non-toxic" TCM(such as Dictamni Cortex). This has aroused the concern of scholars. This study aims to explore the metabolomic mechanism underlying the difference in liver injury induced by dictamnine between males and females through the experiment on 4-week-old mice. The results showed that the serum biochemical indexes of liver function and organ coefficients were significantly increased by dictamnine(P<0.05), and hepatic alveolar steatosis was mainly observed in female mice. However, no histopathological changes were observed in the male mice. Furthermore, a total of 48 differential metabolites(such as tryptophan, corticosterone, and indole) related to the difference in liver injury between males and females were screened out by untargeted metabolomics and multivariate statistical analysis. According to the receiver operating characteristic(ROC) curve, 14 metabolites were highly correlated with the difference. Finally, pathway enrichment analysis indicated that disorders of metabolic pathways, such as tryptophan metabolism, steroid hormone biosynthesis, and ferroptosis(linoleic acid metabolism and arachidonic acid metabolism), may be the potential mechanism of the difference. Liver injury induced by dictamnine is significantly different between males and females, which may be caused by the disorders of tryptophan metabolism, steroid hormone biosynthesis, and ferroptosis pathways.
