Quality evaluation of Huocao based on UPLC fingerprint and multi-component content determination.
10.19540/j.cnki.cjcmm.20230218.201
- Author:
Zheng-Ming YANG
1
;
Ci-Ga DIJIU
2
;
Jian-Long LAN
2
;
Jiang LUO
2
;
Yue-Bu HAILAI
2
;
Tao WANG
2
;
Wen-Bing LI
1
;
Ying LI
2
;
Yuan LIU
3
Author Information
1. Institute of Qinghai-Tibetan Plateau, Southwest Minzu University Chengdu 610225, China Sichuan Provincial Qiang-Yi Medicinal Resources Protection and Utilization Technology Engineering Laboratory Chengdu 610225, China Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization Key Laboratory of National Ethnic Affairs Commission of the People's Republic of China Chengdu 610225, China.
2. College of Pharmacy,Southwest Minzu University Chengdu 610225, China Sichuan Provincial Qiang-Yi Medicinal Resources Protection and Utilization Technology Engineering Laboratory Chengdu 610225, China Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization Key Laboratory of National Ethnic Affairs Commission of the People's Republic of China Chengdu 610225, China.
3. Institute of Qinghai-Tibetan Plateau, Southwest Minzu University Chengdu 610225, China Sichuan Provincial Qiang-Yi Medicinal Resources Protection and Utilization Technology Engineering Laboratory Chengdu 610225, China Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization Key Laboratory of National Ethnic Affairs Commission of the People's Republic of China Chengdu 610225, China Ethnic Medicine Institute, Southwest Minzu University Chengdu 610225, China.
- Publication Type:Journal Article
- Keywords:
Anaphalis margaritacea;
Huocao;
Leontopodium andersonii;
Leontopodium wilsonii;
content determination;
fingerprint;
multivariate statistical analysis
- MeSH:
Chlorogenic Acid;
Entropy;
Hydroxybenzoates;
Quality Control
- From:
China Journal of Chinese Materia Medica
2023;48(11):3000-3013
- CountryChina
- Language:Chinese
-
Abstract:
Huocao(a traditional Chinese herbal medicine) moxibustion is a characteristic technology in Yi medicine suitable for cold-dampness diseases. Huocao, as the moxibustion material, is confusedly used in clinical practice and little is known about its quality control. In this study, UPLC method was used to establish the chemical fingerprint of non-volatile components in Huocao, and the contents of eight phenolic acids such as chlorogenic acid were determined. Multivariate statistical analysis was performed to obtain the indicator components of Huocao for quality evaluation, and thus a comprehensive evaluation system for the quality of Huocao was built. The UPLC fingerprints of 49 batches of Huocao were established, and there were 20 common peaks, of which eight phenolic acids including neochlorogenic acid and chlorogenic acid were identified. Except for three batches of Huocao, the similarity of the other 46 batches was higher than 0.89, suggesting that the established fingerprint method could be used for quality control of the medicinal herb. The correlation coefficient between entropy weight score of the eight phenolic acids and comprehensive fingerprint score in Huocao was 0.875(P<0.01), which indicated that the eight phenolic acids could be used as indicator components for the quality evaluation of Huocao. Furthermore, in multivariate statistical analysis on the common peaks of fingerprint and the contents of the eight phenolic acids, chlorogenic acid, isochlorogenic acid A and isochlorogenic acid C were screened to be the indicator components. The results revealed that the proposed method achieved a simple and accurate quality control of Huocao based on UPLC fingerprint and multi-component content determination, which provided useful data for establishing the quality standard of Huocao.
