Tissue distribution of Qingfei Paidu Decoction based on HPLC-MS/MS.
10.19540/j.cnki.cjcmm.20230123.502
- Author:
Yan ZHANG
1
;
Hai-Yu ZHAO
1
;
Li-Xin YANG
1
;
Yan-Yan ZHOU
1
;
Bao-Lin BIAN
1
;
Hua-Kai WU
2
;
Hua-Ying ZHU
3
;
Nan SI
1
;
Peng-Fei LIN
3
;
Liang WANG
3
;
Hong-Jie WANG
1
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
2. Zhangzhou Pien Tze Huang Pharmaceutical Co., Ltd. Zhangzhou 363000, China.
3. China Resources Sanjiu Medical & Pharmaceutical Co., Ltd. Shenzhen 518110, China.
- Publication Type:Journal Article
- Keywords:
HPLC-MS/MS;
Qingfei Paidu Decoction;
tissue distribution
- MeSH:
Chromatography, High Pressure Liquid;
Tandem Mass Spectrometry;
Tissue Distribution;
Drugs, Chinese Herbal
- From:
China Journal of Chinese Materia Medica
2023;48(11):3074-3085
- CountryChina
- Language:Chinese
-
Abstract:
The tissue distribution of Qingfei Paidu Decoction was studied by HPLC-MS/MS in vivo. Hypersil GOLD C_(18) column(2.1 mm×50 mm, 1.9 μm) was used for gradient elution with acetonitrile as the mobile phase A and 0.1% formic acid solution as the mobile phase B. High-resolution liquid chromatography-mass spectrometry in both positive and negative ion scanning mode and multiple response monitoring(MRM) mode was employed to analyze the behaviors of the active components of Qingfei Paidu Decoction in diffe-rent tissues. The results showed that 19, 9, 17, 14, 22, 19, 24, and 2 compounds were detected in plasma, heart, liver, spleen, lung, kidney, large intestine, and brain, respectively. The compounds belonged to 8 groups, covering 14 herbs in the prescription. After administration with Qingfei Paidu Decoction, the compounds were rapidly distributed in various tissues, especially in the lung, liver, large intestine, and kidney. The majority of the compounds displayed secondary distribution. This study comprehensively analyzed the distribution rules of the main active components in Qingfei Paidu Decoction and provided a basis for the clinical application.
