Species identification of Ligustrum lucidum.
10.19540/j.cnki.cjcmm.20230315.101
- Author:
Yu-Shuang WANG
1
;
Yuan-Xi JIN
1
;
Kang-Jia LIU
1
;
Chang GUO
1
;
Yi-Heng WANG
2
;
Chao XU
3
;
Zhi-Xiang ZHANG
1
;
Wen-Pan DONG
1
Author Information
1. School of Ecology and Nature Conservation, Beijing Forestry University Beijing 100083, China.
2. State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
3. State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany,Chinese Academy of Sciences Beijing 100093, China.
- Publication Type:Journal Article
- Keywords:
DNA barcode;
DNA extraction;
Ligustrum lucidum;
species identification
- MeSH:
Ligustrum/genetics*;
Seeds;
Fruit;
Polymerase Chain Reaction;
Research Design
- From:
China Journal of Chinese Materia Medica
2023;48(11):2940-2948
- CountryChina
- Language:Chinese
-
Abstract:
Ligustrum lucidum is a woody perennial plant of genus Ligustrum in family Oleaceae. Its dried fruit has high medicinal value. In this study, the authors evaluated the variability and species identification efficiency of three specific DAN barcodes(rbcL-accD, ycf1a, ycf1b) and four general DAN barcodes(matK, rbcL, trnH-psbA, ITS2) for a rapid and accurate molecular identification of Ligustrum species. The results revealed that matK, rbcL, trnH-psbA, ITS2 and ycf1a were inefficient for identifying the Ligustrum species, and a large number of insertions and deletions were observed in rbcL-accD sequence, which was thus unsuitable for development as specific barcode. The ycf1b-2 barcode had DNA barcoding gap and high success rate of PCR amplification and DNA sequencing, which was the most suitable DNA barcode for L. lucidum identification and achieved an accurate result. In addition, to optimize the DNA extraction experiment, the authors extracted and analyzed the DNA of the exocarp, mesocarp, endocarp and seed of L. lucidum fruit. It was found that seed was the most effective part for DNA extraction, where DNAs of high concentration and quality were obtained, meeting the needs of species identification. In this study, the experimental method for DNA extraction of L. lucidum was optimized, and the seed was determined as the optimal part for DNA extraction and ycf1b-2 was the specific DNA barcode for L. lucidum identification. This study laid a foundation for the market regulation of L. lucidum.
