Immune regulation mechanism of Saposhnikoviae Radix polysaccharide based on zebrafish model.
10.19540/j.cnki.cjcmm.20221201.702
- Author:
Meng SUN
1
;
Wen-di WANG
1
;
Yan LI
1
;
Ke-Chun LIU
2
;
Qing XIA
2
;
Yan-Yan JIANG
3
;
Bin LIU
3
Author Information
1. School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China.
2. Biology Institute, Qilu University of Technology (Shandong Academy of Sciences) Ji'nan 250103, China.
3. School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China Key Laboratory of "Discovery of Effective Substances in Classical Prescriptions of Traditional Chinese Medicine",State Administration of Traditional Chinese Medicine Beijing 102488, China.
- Publication Type:Journal Article
- Keywords:
Saposhnikoviae Radix;
immunomodulation;
polysaccharide;
transcription sequencing;
zebrafish
- MeSH:
Animals;
Zebrafish/genetics*;
Interleukin-6/genetics*;
Gene Expression Profiling;
Cytokines/genetics*;
Macrophages;
Transcriptome
- From:
China Journal of Chinese Materia Medica
2023;48(7):1916-1926
- CountryChina
- Language:Chinese
-
Abstract:
The immunomodulatory effect of Saposhnikoviae Radix polysaccharide(SRP) was evaluated based on the zebrafish mo-del, and its mechanism was explored by transcriptome sequencing and real-time fluorescence-based quantitative PCR(RT-qPCR). The immune-compromised model was induced by navelbine in the immunofluorescence-labeled transgenic zebrafish Tg(lyz: DsRed), and the effect of SRP on the density and distribution of macrophages in zebrafish was evaluated. The effect of SRP on the numbers of macrophages and neutrophils in wild-type AB zebrafish was detected by neutral red and Sudan black B staining. The content of NO in zebrafish was detected by DAF-FM DA fluorescence probe. The content of IL-1β and IL-6 in zebrafish was detected by ELISA. The differentially expressed genes(DEGs) of zebrafish in the blank control group, the model group, and the SRP treatment group were analyzed by transcriptome sequencing. The immune regulation mechanism was analyzed by Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment, and the expression levels of key genes were verified by RT-qPCR. The results showed that SRP could significantly increase the density of immune cells in zebrafish, increase the number of macrophages and neutrophils, and reduce the content of NO, IL-1β, and IL-6 in immune-compromised zebrafish. The results of transcriptome sequencing analysis showed that SRP could affect the expression level of immune-related genes on Toll-like receptor pathway and herpes simplex infection pathway to affect the release of downstream cytokines and interferon, thereby completing the activation process of T cells and playing a role in regulating the immune activity of the body.
