Content determination of ten flavonoids and alkaloids in Gleditsiae Sinensis Fructus, Gleditsiae Fructus Abnormalis, and Gleditsiae Spina.
10.19540/j.cnki.cjcmm.20221228.201
- Author:
Hu-Min XIE
1
;
Yue-Guang MI
1
;
Xiao-Yan XU
1
;
Meng-Xiao SUN
1
;
Er-Wei LIU
1
;
Xiu-Mei GAO
1
;
Xue LI
1
;
Wen-Zhi YANG
1
Author Information
1. State Key Laboratory of Component-based Chinese Medicines, Tianjin University of Traditional Chinese Medicine Tianjin 301617, China.
- Publication Type:Journal Article
- Keywords:
Gleditsiae Fructus Abnormalis;
Gleditsiae Sinensis Fructus;
Gleditsiae Spina;
UHPLC-Q-Trap-MS;
alkaloid;
flavonoid;
multiple reaction monitoring
- MeSH:
Flavonoids/analysis*;
Alkaloids;
Chromatography, High Pressure Liquid/methods*;
Mass Spectrometry;
Drugs, Chinese Herbal
- From:
China Journal of Chinese Materia Medica
2023;48(7):1899-1907
- CountryChina
- Language:Chinese
-
Abstract:
To study the quality control of three traditional Chinese medicines derived from Gleditsia sinensis [Gleditsiae Sinensis Fructus(GSF), Gleditsiae Fructus Abnormalis(GFA), and Gleditsiae Spina(GS)], this paper established a multiple reaction monitoring(MRM) approach based on ultra-high performance liquid chromatography-triple quadrupole-linear ion-trap mass spectrometry(UHPLC-Q-Trap-MS). Using an ACQUITY UPLC BEH C_(18) column(2.1 mm × 100 mm, 1.7 μm), gradient elution was performed at 40 ℃ with water containing 0.1% formic acid-acetonitrile as the mobile phase running at 0.3 mL·min~(-1), and the separation and content determination of ten chemical constituents(e.g., saikachinoside A, locustoside A, orientin, taxifolin, vitexin, isoquercitrin, luteolin, quercitrin, quercetin, and apigenin) in GSF, GFA, and GS were enabled within 31 min. The established method could quickly and efficiently determine the content of ten chemical constituents in GSF, GFA, and GS. All constituents showed good linearity(r>0.995), and the average recovery rate was 94.09%-110.9%. The results showed that, the content of two alkaloids in GSF(2.03-834.75 μg·g~(-1)) was higher than that in GFA(0.03-10.41 μg·g~(-1)) and GS(0.04-13.66 μg·g~(-1)), while the content of eight flavonoids in GS(0.54-2.38 mg·g~(-1)) was higher than that in GSF(0.08-0.29 mg·g~(-1)) and GFA(0.15-0.32 mg·g~(-1)). These results provide references for the quality control of G. sinensis-derived TCMs.
