Optimization of extraction process of Chuanxiong Rhizoma-Gastrodiae Rhizoma based on network pharmacology and multi-index orthogonal test.
10.19540/j.cnki.cjcmm.20221125.301
- Author:
Yu-Tong QI
1
;
Miao ZHANG
1
;
Shuo MENG
1
;
Jun-Guo REN
1
;
Jian-Xun LIU
1
Author Information
1. Beijing Key Laboratory of Pharmacology of Chinese Materia Medica, Institute of Basic Medical Sciences of Xiyuan Hospital,China Academy of Chinese Medical Sciences Beijing 100091, China.
- Publication Type:Journal Article
- Keywords:
Chuanxiong Rhizoma-Gastrodiae Rhizoma herb pair;
extraction process;
network pharmacology;
orthogonal test
- MeSH:
Drugs, Chinese Herbal/pharmacology*;
Network Pharmacology;
Molecular Docking Simulation;
Rhizome
- From:
China Journal of Chinese Materia Medica
2023;48(7):1858-1865
- CountryChina
- Language:Chinese
-
Abstract:
To optimize the extraction process of Chuanxiong Rhizoma-Gastrodiae Rhizoma herb pair by network pharmacology combined with analytic hierarchy process(AHP)-entropy weight method and multi-index orthogonal test. The potential active components and targets of Chuanxiong Rhizoma-Gastrodiae Rhizoma were screened by network pharmacology and molecular docking, and the process evaluation indexes were determined with reference to the Chinese Pharmacopoeia(2020 edition). The core components of Chuanxiong Rhizoma-Gastrodiae Rhizoma were determined as gastrodin, parishin B, parishin C, parishin E, ferulic acid, and 3-butylphthalide. With the extraction volume of each indicator and yield of dry extract as comprehensive evaluation indicators, the extraction conditions were optimized by the AHP-entropy weight method and orthogonal test as the ethanol volume of 50%, the solid-liquid ratio of 1∶8(g·mL~(-1)), extraction for three times, and 1.5 h each time. Through network pharmacology and molecular docking, the process evaluation index was determined, and the optimized process was stable and reproducible for the extraction of Chuanxiong Rhizoma-Gastrodiae Rhizoma herb pair, which could provide reference for in-depth research.
