Potentiating effect and mechanism of extract of Jingfang Granules on activation of macrophages.
10.19540/j.cnki.cjcmm.20230131.401
- Author:
Dou-Dou HAO
1
;
Zi-Han LU
1
;
Yang-Gan LUO
1
;
Peng-Fei TU
2
;
Cheng-Hong SUN
3
;
Jing-Chun YAO
3
;
Qing WU
1
;
Zhi-Xiang ZHU
1
Author Information
1. School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 100029, China.
2. State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Beijing 100191, China.
3. State Key Laboratory of Generic Manufacture Technology of Chinese Traditional Medicine,Lunan Pharmaceutical Group Co., Ltd. Linyi 276004, China.
- Publication Type:Journal Article
- Keywords:
Jingfang Granules;
cytokines;
immunoenhancement;
innate immunity;
macrophages
- MeSH:
Tumor Necrosis Factor-alpha/metabolism*;
Interleukin-6/metabolism*;
Plant Extracts/metabolism*;
Lipopolysaccharides/pharmacology*;
Macrophages;
Cytokines/metabolism*;
RNA, Messenger/metabolism*
- From:
China Journal of Chinese Materia Medica
2023;48(10):2803-2809
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed to explore the potentiating effect and mechanism of the extract of Jingfang Granules(JFG) on the activation of macrophages. The RAW264.7 cells were treated with JFG extract and then stimulated by multiple agents. Subsequently, mRNA was extracted, and reverse transcription-polymerase chain reaction(RT-PCR) was used to measure the mRNA transcription of multiple cytokines in RAW264.7 cells. The levels of cytokines in the cell supernatant were detected by enzyme-linked immunosorbent assay(ELISA). In addition, the intracellular proteins were extracted and the activation of signaling pathways was determined by Western blot. The results showed that JFG extract alone could not promote or slightly promote the mRNA transcription of TNF-α, IL-6, IL-1β, MIP-1α, MCP-1, CCL5, IP-10, and IFN-β, and significantly enhance the mRNA transcription of these cytokines in RAW264.7 cells induced by R848 and CpG in a dose-dependent manner. Furthermore, JFG extract also potentiated the secretion of TNF-α, IL-6, MCP-1, and IFN-β by RAW264.7 cells stimulated with R848 and CpG. As revealed by mechanism analysis, JFG extract enhanced the phosphorylation of p38, ERK1/2, IRF3, STAT1, and STAT3 in RAW264.7 cells induced by CpG. The findings of this study indicate that JFG extract can selectively potentiate the activation of macrophages induced by R848 and CpG, which may be attributed to the promotion of the activation of MAPKs, IRF3, and STAT1/3 signaling pathways.
