Mechanism of tryptanthrin in treatment of ulcerative colitis in mice based on serum metabolomics.
10.19540/j.cnki.cjcmm.20221213.401
- Author:
Jie ZHU
1
;
Bao-Long HOU
1
;
Wen CHENG
1
;
Ting WANG
1
;
Zheng WANG
1
;
Yan-Ni LIANG
1
Author Information
1. Co-construction Collaborative Innovation Center for Chinese Medicine Resources Industrialization by Shaanxi & Education Ministry/State Key Laboratory of Research & Development of Characteristic Qin Medicine Resources (Cultivation)/Shaanxi Innovative Drug Research Center, Shaanxi University of Chinese Medicine Xianyang 712083, China.
- Publication Type:Journal Article
- Keywords:
arachidonic acid metabolism;
purine metabolism;
serum metabolomics;
tryptanthrin;
tryptophan metabolism;
ulcerative colitis
- MeSH:
Mice;
Animals;
Colitis, Ulcerative/drug therapy*;
Tryptophan;
Arachidonic Acid/metabolism*;
Mice, Inbred C57BL;
Colon;
Cytokines/metabolism*;
Tumor Necrosis Factor-alpha/metabolism*;
Interleukin-6/metabolism*;
Metabolomics;
Purines/therapeutic use*;
Dextran Sulfate/metabolism*;
Disease Models, Animal;
Colitis/chemically induced*
- From:
China Journal of Chinese Materia Medica
2023;48(8):2193-2202
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to explore the effect of tryptanthrin on potential metabolic biomarkers in the serum of mice with ulcerative colitis(UC) induced by dextran sulfate sodium(DSS) based on liquid chromatography-mass spectrometry(LC-MS) and predict the related metabolic pathways. C57BL/6 mice were randomly assigned into a tryptanthrin group, a sulfasalazine group, a control group, and a model group. The mouse model of UC was established by free drinking of 3% DSS solution for 11 days, and corresponding drugs were adminsitrated at the same time. The signs of mice were observed and the disease activity index(DAI) score was recorded from the first day. Colon tissue samples were collected after the experiment and observed by hematoxylin-eosin(HE) staining. The levels of interleukin-4(IL-4), interleukin-10(IL-10), tumor necrosis factor-α(TNF-α), interleukin-6(IL-6), and interleukin-8(IL-8) in the serum were measured by enzyme linked immunosorbent assay(ELISA). The serum samples were collected from 6 mice in each group for widely targeted metabolomics. The metabolic pathways were enriched by MetaboAnalyst 5.0. The results showed that compared with the model group, tryptanthrin treatment decreased the DAI score(P<0.05), alleviated the injury of the colon tissue and the infiltration of inflammatory cells, lowered the levels of proinflammatory cytokines, and elevated the levels of anti-inflammatory cytokines in the serum. The metabolomic analysis revealed 28 differential metabolites which were involved in 3 metabolic pathways including purine metabolism, arachidonic acid metabolism, and tryptophan metabolism. Tryptanthrin may restore the metabolism of the mice with UC induced by DSS to the normal level by regulating the purine metabolism, arachidonic acid metabolism, and tryptophan metabolism. This study employed metabolomics to analyze the mechanism of tryptanthrin in the treatment of UC, providing an experimental basis for the utilization and development of tryptanthrin.
