Leonurine inhibits ferroptosis in renal tubular epithelial cells by activating p62/Nrf2/HO-1 signaling pathway.
10.19540/j.cnki.cjcmm.20221115.401
- Author:
Ai-Jun WU
1
;
Nai-Qing CHEN
1
;
Li-Hua HUANG
2
;
Ran CHENG
1
;
Xiao-Wan WANG
2
;
Chuang LI
2
;
Wei MAO
2
;
Qing-Ming HUANG
1
;
Peng XU
2
;
Rui-Min TIAN
3
Author Information
1. State Key Laboratory of Dampness Syndrome of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine & Guangdong Provincial Hospital of Chinese Medicine Guangzhou 510120, China the Second Clinical Medical College of Guangzhou University of Chinese Medicine Guangzhou 510405, China.
2. State Key Laboratory of Dampness Syndrome of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine & Guangdong Provincial Hospital of Chinese Medicine Guangzhou 510120, China the Second Clinical Medical College of Guangzhou University of Chinese Medicine Guangzhou 510405, China Guangdong Provincial Academy of Chinese Medical Sciences Guangzhou 510006, China.
3. State Key Laboratory of Dampness Syndrome of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine & Guangdong Provincial Hospital of Chinese Medicine Guangzhou 510120, China the Second Clinical Medical College of Guangzhou University of Chinese Medicine Guangzhou 510405, China Guangdong Provincial Academy of Chinese Medical Sciences Guangzhou 510006, China Faculty of Chinese Medicine, Macau University of Science and Technology Macao 999078, China.
- Publication Type:Journal Article
- Keywords:
ferroptosis;
heme oxy-genase 1(HO-1);
leonurine;
nuclear factor erythroid 2-related factor 2(Nrf2);
p62;
renal tubular epithelial cells
- MeSH:
Humans;
Ferroptosis;
Reactive Oxygen Species/metabolism*;
NF-E2-Related Factor 2/metabolism*;
Sincalide/pharmacology*;
Signal Transduction;
Epithelial Cells/metabolism*;
Glutathione
- From:
China Journal of Chinese Materia Medica
2023;48(8):2176-2183
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the protective effect and the potential mechanism of leonurine(Leo) against erastin-induced ferroptosis in human renal tubular epithelial cells(HK-2 cells), an in vitro erastin-induced ferroptosis model was constructed to detect the cell viability as well as the expressions of ferroptosis-related indexes and signaling pathway-related proteins. HK-2 cells were cultured in vitro, and the effects of Leo on the viability of HK-2 cells at 10, 20, 40, 60, 80 and 100 μmol·L~(-1) were examined by CCK-8 assay to determine the safe dose range of Leo administration. A ferroptosis cell model was induced by erastin, a common ferroptosis inducer, and the appropriate concentrations were screened. CCK-8 assay was used to detect the effects of Leo(20, 40, 80 μmol·L~(-1)) and positive drug ferrostatin-1(Fer-1, 1, 2 μmol·L~(-1)) on the viability of ferroptosis model cells, and the changes of cell morphology were observed by phase contrast microscopy. Then, the optimal concentration of Leo was obtained by Western blot for nuclear factor erythroid 2-related factor 2(Nrf2) activation, and transmission electron microscope was further used to detect the characteristic microscopic morphological changes during ferroptosis. Flow cytometry was performed to detect reactive oxygen species(ROS), and the level of glutathione(GSH) was measured using a GSH assay kit. The expressions of glutathione peroxidase 4(GPX4), p62, and heme oxygenase 1(HO-1) in each group were quantified by Western blot. RESULTS:: showed that Leo had no side effects on the viability of normal HK-2 cells in the concentration range of 10-100 μmol·L~(-1). The viability of HK-2 cells decreased as the concentration of erastin increased, and 5 μmol·L~(-1) erastin significantly induced ferroptosis in the cells. Compared with the model group, Leo dose-dependently increased cell via-bility and improved cell morphology, and 80 μmol·L~(-1) Leo promoted the translocation of Nrf2 from the cytoplasm to the nucleus. Further studies revealed that Leo remarkably alleviated the characteristic microstructural damage of ferroptosis cells caused by erastin, inhibited the release of intracellular ROS, elevated GSH and GPX4, promoted the nuclear translocation of Nrf2, and significantly upregulated the expression of p62 and HO-1 proteins. In conclusion, Leo exerted a protective effect on erastin-induced ferroptosis in HK-2 cells, which might be associated with its anti-oxidative stress by activating p62/Nrf2/HO-1 signaling pathway.
