Separation, characterization and anti-psoriasis effect of self-assembled nanoparticles from Shaoyao Gancao Decoction.
10.19540/j.cnki.cjcmm.20221223.301
- Author:
Zhi-Jian QIN
1
;
Qiao YAN
2
;
Ling-Yu HANG
2
;
Xiao-Han TANG
1
;
Fang-Qin LI
2
;
Yu-Ye XUE
2
;
Hai-Long YUAN
3
Author Information
1. School of Pharmacy, Anhui Medical University Hefei 230032, China.
2. Department of Pharmacy,Air Force Medical Center, PLA Beijing 100142, China.
3. School of Pharmacy, Anhui Medical University Hefei 230032, China Department of Pharmacy,Air Force Medical Center, PLA Beijing 100142, China.
- Publication Type:Journal Article
- Keywords:
Shaoyao Gancao Decoction;
determination of content;
mechanism of action;
psoriasis;
self-assembled nanoparticles;
separation and characterization;
substance basis
- MeSH:
Mice;
Animals;
Imiquimod;
Drugs, Chinese Herbal/pharmacology*;
Glycyrrhizic Acid;
Chromatography, High Pressure Liquid/methods*
- From:
China Journal of Chinese Materia Medica
2023;48(8):2116-2125
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to separate and characterize self-assembled nanoparticles(SAN) from Shaoyao Gancao Decoction(SGD) and determine the content of active compounds. Further, we aimed to observe the therapeutic effect of SGD-SAN on imiquimod-induced psoriasis in mice. The separation of SGD was performed by dialysis, and the separation process was optimized by single factor experiment. The SGD-SAN isolated under the optimal process was characterized, and the content of gallic acid, albiflorin, paeoniflorin, liquiritin, isoliquiritin apioside, isoliquiritin, and glycyrrhizic acid in each part of SGD was determined by HPLC. In the animal experiment, mice were assigned into a normal group, a model group, a methotrexate group(0.001 g·kg~(-1)), and SGD, SGD sediment, SGD dialysate, and SGD-SAN groups of different doses(1, 2, and 4 g·kg~(-1)) respectively. The psoriasis grade of mice was evaluated based on the pathological changes of skin lesions, the content of inflammatory cytokines, organ index and other indicators. The results showed that SAN obtained by centrifugation at 13 000 r·min~(-1) for 30 min was stable after dialysis for 4 times, which were uniform spherical nanoparticles with the particle size of(164.43±1.34) nm, the polydispersity index of(0.28±0.05), and the Zeta potential of(-12.35±0.80) mV. The active compound content accounted for more than 70% of SGD. Compared with the model group, SAN and SGD decreased the skin lesion score, spleen index, and inflammatory cytokine levels(P<0.05 or P<0.01) and alleviated the skin thickening and infiltration of inflammatory cells. However, the sediment group and the dialysate group had no obvious effect. SGD showed a good therapeutic effect on imiquimod-induced psoriasis in mice, and SAN demonstrated the effect equivalent to SGD in a dose-dependent manner. Therefore, we conclude that the SAN formed during decocting is the main active form of SGD, which can lower the levels of inflammatory cytokines, promote the normal differentiation of keratinocytes, and reduce the infiltration of inflammatory cells in the treatment of psoriasis lesions in mice.
