Mining and identification of members of MYB transcription factor family in Lonicera macranthoides.
10.19540/j.cnki.cjcmm.20230115.103
- Author:
Juan ZENG
1
;
Yu-Qing LONG
1
;
Xue-Sen FU
1
;
Ling WANG
1
;
Zi-Xuan LIU
1
;
Ri-Bao ZHOU
2
;
Xiang-Dan LIU
2
Author Information
1. School of Pharmacy, Hunan University of Chinese Medicine Changsha 410208, China Key Laboratory of Germplasm Resources and Standardized Planting of Bulk Authentic Medicinal Materials Changsha 410208, China.
2. School of Pharmacy, Hunan University of Chinese Medicine Changsha 410208, China Key Laboratory of Germplasm Resources and Standardized Planting of Bulk Authentic Medicinal Materials Changsha 410208, China Key Laboratory of Traditional Chinese Medicine Modernization Research in General Colleges and Universities of Hunan Province Changsha 410208, China.
- Publication Type:Journal Article
- Keywords:
Lonicera macranthoides;
MYB;
bioinformatics;
transcription factor
- MeSH:
Transcription Factors/metabolism*;
Lonicera/metabolism*;
Phylogeny;
Plant Proteins/metabolism*;
Gene Expression Regulation, Plant
- From:
China Journal of Chinese Materia Medica
2023;48(8):2103-2115
- CountryChina
- Language:Chinese
-
Abstract:
As a large family of transcription factors, the MYB family plays a vital role in regulating flower development. We studied the MYB family members in Lonicera macranthoides for the first time and identified three sequences of 1R-MYB, 47 sequences of R2R3-MYB, two sequences of 3R-MYB, and one sequence of 4R-MYB from the transcriptome data. Further, their physicochemical properties, conserved domains, phylogenetic relationship, protein structure, functional information, and expression were analyzed. The results show that the 53 MYB transcription factors had different conserved motifs, physicochemical properties, structures, and functions in wild type and 'Xianglei' cultivar of L. macranthoides, indicating their conservation and diversity in evolution. The transcript level of LmMYB was significantly different between the wild type and 'Xianglei' cultivar as well as between flowers and leaves, and some genes were specifically expressed. Forty-three out of 53 LmMYB sequences were expressed in both flowers and leaves, and 9 of the LmMYB members showed significantly different transcript levels between the wild type and 'Xianglei' cultivar, which were up-regulated in the wild type. The results provide a theoretical basis for further studying the specific functional mechanism of the MYB family.
