Application of high-speed counter current chromatography in extraction and separation of alkaloids in natural products.
10.19540/j.cnki.cjcmm.20221207.601
- Author:
Hao-Hao GUO
1
;
Jing WANG
1
;
Yuan CHEN
1
;
Xiu-Lan XIN
2
;
Yu-Feng LIU
1
Author Information
1. School of Pharmaceutical Sciences, Liaoning University Shenyang 110036, China.
2. College of Bioengineering,Beijing Polytechnic Beijing 100176, China.
- Publication Type:Journal Article
- Keywords:
alkaloids;
elution mode;
extraction and separation;
high-speed counter current chromatography;
solvent system
- MeSH:
Biological Products;
Countercurrent Distribution/methods*;
Chromatography, High Pressure Liquid/methods*;
Alkaloids/analysis*;
Solvents/chemistry*
- From:
China Journal of Chinese Materia Medica
2023;48(8):1989-1999
- CountryChina
- Language:Chinese
-
Abstract:
Alkaloids, widespread in plants, have a series of pharmacological activities and have been widely used to treat various diseases. Because alkaloids are usually presented in multicomponent mixtures and are deeply low in content, they are very difficult to extract and separate by traditional methods. High-speed counter current chromatography(HSCCC) is a kind of liquid-liquid chromatography without solid support phase, which has the advantages of large injection volume, low cost, and no irreversible adsorption. Compared with the traditional methods of extraction and separation of alkaloids, HSCCC can ensure the separation of many different alkaloids at one time, with a high recovery and large amount. In this paper, the advantages and disadvantages of HSCCC compared with traditional separation methods were discussed and the solvent system and elution mode of HSCCC used to separate alkaloids in recent years were summarized by referring to the relevant literature to provide some references for the separation of alkaloids by HSCCC.
