Metabolomics study of Berberidis Radix in intervening ulcerative colitis based on UPLC-Q-TOF-MS.
10.19540/j.cnki.cjcmm.20230130.401
- Author:
Xue-Li HU
1
;
Chang-Yuan ZHOU
2
;
Rui XU
3
;
Hong LI
4
;
Bao YANG
5
;
Jian LONG
3
;
Xing TU
6
;
Juan NIE
4
;
Ke-Yun LIU
3
;
Ze-Hua HU
7
Author Information
1. Hubei Key Laboratory of Biological Resources Protection and Utilization, Hubei Minzu University Enshi 445000, China Hubei Key Laboratory of Occurrence and Intervention of Rheumatic Diseases, Hubei Minzu University Enshi 445000, China Medical School, Hubei Minzu University Enshi 445000, China.
2. Hubei Key Laboratory of Occurrence and Intervention of Rheumatic Diseases, Hubei Minzu University Enshi 445000, China.
3. Medical School, Hubei Minzu University Enshi 445000, China.
4. Medical School, Hubei Minzu University Enshi 445000, China Chinese Medicinal Materials Products Quality Supervision and Inspection Center in Wuling Mountainous Area, Hubei Minzu University Enshi 445000, China.
5. Hubei Key Laboratory of Occurrence and Intervention of Rheumatic Diseases, Hubei Minzu University Enshi 445000, China Medical School, Hubei Minzu University Enshi 445000, China Chinese Medicinal Materials Products Quality Supervision and Inspection Center in Wuling Mountainous Area, Hubei Minzu University Enshi 445000, China.
6. Chinese Medicinal Materials Products Quality Supervision and Inspection Center in Wuling Mountainous Area, Hubei Minzu University Enshi 445000, China.
7. Hubei Key Laboratory of Biological Resources Protection and Utilization, Hubei Minzu University Enshi 445000, China Hubei Key Laboratory of Occurrence and Intervention of Rheumatic Diseases, Hubei Minzu University Enshi 445000, China Medical School, Hubei Minzu University Enshi 445000, China Chinese Medicinal Materials Products Quality Supervision and Inspection Center in Wuling Mountainous Area, Hubei Minzu University Enshi 445000, China.
- Publication Type:Journal Article
- Keywords:
Berberidis Radix;
Tujia medicine;
UPLC-Q-TOF-MS;
metabolomics;
ulcerative colitis
- MeSH:
Mice;
Animals;
Colitis, Ulcerative/drug therapy*;
Interleukin-10;
Metabolomics/methods*;
Chromatography, High Pressure Liquid
- From:
China Journal of Chinese Materia Medica
2023;48(9):2490-2499
- CountryChina
- Language:Chinese
-
Abstract:
The effect of Tujia medicine Berberidis Radix on endogenous metabolites in the serum and feces of mice with ulcerative colitis(UC) induced by dextran sulfate sodium(DSS) was analyzed by metabolomics technology to explore the metabolic pathway and underlying mechanism of Berberidis Radix in the intervention of UC. The UC model was induced in mice by DSS. Body weight, disease activity index(DAI), and colon length were recorded. The levels of tumor necrosis factor-α(TNF-α) and interleukin-10(IL-10) in colon tissues were determined by ELISA. The levels of endogenous metabolites in the serum and feces were detected by ultra-high-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS). Principal component analysis(PCA) and orthogonal partial least squares-discriminant analysis(OPLS-DA) were employed to characterize and screen differential metabolites. The potential metabolic pathways were analyzed by MetaboAnalyst 5.0. The results showed that Berberidis Radix could significantly improve the symptoms of UC mice and increase the level of the anti-inflammatory factor IL-10. A total of 56 and 43 differential metabolites were identified in the serum and feces, respectively, belonging to lipids, amino acids, fatty acids, etc. After the intervention by Berberidis Radix, the metabolic disorder gradually recovered. The involved metabolic pathways included biosynthesis of phenylalanine, tyrosine, and tryptophan, linoleic acid metabolism, phenylalanine metabolism, and glycerophospholipid metabolism. Berberidis Radix can alleviate the symptoms of mice with DSS-induced UC, and the mechanism may be closely related to the re-gulation of lipid metabolism, amino acid metabolism, and energy metabolism.
