LC-MS analysis of 2-(2-phenylethyl) chromones in sodium chloride-treated suspension cells of Aquilaria sinensis.
10.19540/j.cnki.cjcmm.20230208.201
- Author:
Yu DU
1
;
Xiao-Xue ZHANG
1
;
Ze-Kun ZHANG
1
;
Wen-Jing WANG
1
;
Bei-Bei ZHANG
1
;
Ming-Liang ZHANG
1
;
Yang WANG
1
;
Xiang-Yu GE
1
;
She-Po SHI
1
Author Information
1. Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 100029, China.
- Publication Type:Journal Article
- Keywords:
2-(2-phenylethyl) chromones;
Aquilaria sinensis;
Aquilariae Lignum Resinatum;
LC-MS;
suspension cells
- MeSH:
Chromones;
Sodium Chloride;
Chromatography, Liquid;
Flavonoids;
Tandem Mass Spectrometry;
Thymelaeaceae
- From:
China Journal of Chinese Materia Medica
2023;48(9):2480-2489
- CountryChina
- Language:Chinese
-
Abstract:
Qualitative and quantitative analysis of 2-(2-phenylethyl) chromones in sodium chloride(NaCl)-treated suspension cells of Aquilaria sinensis was conducted by UPLC-Q-Exactive-MS and UPLC-QQQ-MS/MS. Both analyses were performed on a Waters T3 column(2.1 mm×50 mm, 1.8 μm) with 0.1% formic acid aqueous solution(A)-acetonitrile(B) as mobile phases at gradient elution. MS data were collected by electrospray ionization in positive ion mode. Forty-seven phenylethylchromones was identified from NaCl-treated suspension cell samples of A. sinensis using UPLC-Q-Exactive-MS, including 22 flindersia-type 2-(2-phenylethyl) chromones and their glycosides, 10 5,6,7,8-tetrahydro-2-(2-phenylethyl) chromones and 15 mono-epoxy or diepoxy-5,6,7,8-tetrahydro-2-(2-phenylethyl) chromones. Additionally, 25 phenylethylchromones were quantitated by UPLC-QQQ-MS/MS. Overall, the rapid and efficient qualitative and quantitative analysis of phenylethylchromones in NaCl-treated suspension cells of A. sinensis by two LC-MS techniques, provides an important reference for the yield of phenylethylchromones in Aquilariae Lignum Resinatum using in vitro culture and other biotechnologies.
