Genetic Subtypes and Pretreatment Drug Resistance in the Newly Reported Human Immunodeficiency Virus-Infected Men Aged≥50 Years Old in Guangxi.
10.3881/j.issn.1000-503X.15444
- Author:
Ning-Ye FANG
1
;
Wen-Cui WEI
2
;
Jian-Jun LI
1
;
Ping CEN
3
;
Xian-Xiang FENG
4
;
Dong YANG
5
;
Kai-Ling TANG
1
;
Shu-Jia LIANG
1
;
Yu-Lan SHAO
2
;
Hua-Xiang LU
1
;
He JIANG
1
;
Qin MENG
1
;
Shuai-Feng LIU
1
;
Qiu-Ying ZHU
1
;
Huan-Huan CHEN
1
;
Guang-Hua LAN
1
;
Shi-Xiong YANG
3
;
Li-Fang ZHOU
4
;
Jing-Lin MO
5
;
Xian-Min GE
1
Author Information
1. Institute of HIV/AIDS Prevention and Control,Guangxi Zhuang Autonomous Region Center for Disease Prevention and Control,Nanning 530028,China.
2. Department of Clinical Examination,Guigang Center for Disease Control and Prevention,Guigang,Guangxi 537100,China.
3. Department of HIV/AIDS/STD Prevention and Control,Nanning Center for Disease Control and Prevention,Nanning 530023,China.
4. Institute of HIV/AIDS Prevention and Control,Liuzhou Center for Disease Control and Prevention,Liuzhou,Guangxi 545007,China.
5. Department of HIV/AIDS/STD Prevention and Control,Guilin Center for Disease Control and Prevention,Guilin,Guangxi 541001,China.
- Publication Type:Journal Article
- Keywords:
genetic subtype;
human immunodeficiency virus;
pretreatment drug resistance
- MeSH:
Male;
Humans;
Middle Aged;
Reverse Transcriptase Inhibitors/therapeutic use*;
HIV Infections/drug therapy*;
Drug Resistance, Viral/genetics*;
China/epidemiology*;
Mutation;
HIV-1/genetics*;
Protease Inhibitors/therapeutic use*;
Genotype
- From:
Acta Academiae Medicinae Sinicae
2023;45(3):399-404
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the genetic subtypes of human immunodeficiency virus (HIV) and the prevalence of pretreatment drug resistance in the newly reported HIV-infected men in Guangxi. Methods The stratified random sampling method was employed to select the newly reported HIV-infected men aged≥50 years old in 14 cities of Guangxi from January to June in 2020.The pol gene of HIV-1 was amplified by nested reverse transcription polymerase chain reaction and then sequenced.The mutation sites associated with drug resistance and the degree of drug resistance were then analyzed. Results A total of 615 HIV-infected men were included in the study.The genetic subtypes of CRF01_AE,CRF07_BC,and CRF08_BC accounted for 57.4% (353/615),17.1% (105/615),and 22.4% (138/615),respectively.The mutations associated with the resistance to nucleoside reverse transcriptase inhibitors (NRTI),non-nucleoside reverse transcriptase inhibitors (NNRTI),and protease inhibitors occurred in 8 (1.3%),18 (2.9%),and 0 patients,respectively.M184V (0.7%) and K103N (1.8%) were the mutations with the highest occurrence rates for the resistance to NRTIs and NNRTIs,respectively.Twenty-two (3.6%) patients were resistant to at least one type of inhibitors.Specifically,4 (0.7%),14 (2.3%),4 (0.7%),and 0 patients were resistant to NRTIs,NNRTIs,both NRTIs and NNRTIs,and protease inhibitors,respectively.The pretreatment resistance to NNRTIs had much higher frequency than that to NRTIs (2.9% vs.1.3%;χ2=3.929,P=0.047).The prevalence of pretreatment resistance to lamivudine,zidovudine,tenofovir,abacavir,rilpivirine,efavirenz,nevirapine,and lopinavir/ritonavir was 0.8%, 0.3%, 0.7%, 1.0%, 1.3%, 2.8%, 2.9%, and 0, respectively. Conclusions CRF01_AE,CRF07_BC,and CRF08_BC are the three major strains of HIV-infected men≥50 years old newly reported in Guangxi,2020,and the pretreatment drug resistance demonstrates low prevalence.
